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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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Original Research Articles                      Volume : 9, Issue:5, May, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 258-270
DOI: https://doi.org/10.20546/ijcmas.2020.905.029


Influence of Green Synthesized Zinc Oxide Nanoparticles on Seed Germination and Seedling Growth in Wheat (Triticum aestivum)
Bhupendra Bhaskar Meher1, Shivraj Sahu1, Shivangi Singhal1, Mayank Joshi1, Poonam Maan2 and Sneh Gautam1*
1Department of Molecular Biology & Genetic Engineering, G.B.Pant University of Agriculture & Technology, Pantnagar-263145, Uttarakhand, India
2Department of Agriculture Biotechnology, S.V.P University of Agriculture & Technology, Meerut-250110, U.P, India
*Corresponding author
Abstract:

In present study, Zinc Oxide nanoparticles (ZnO NPs) were synthesized from leaf extract of Catharanthus roseus by green synthesis method. The synthesized nanoparticles were characterized by UV-visible spectrophotometry and Dynamic light spectroscopy (DLS) that showed the synthesis of nanoparticles of average size 385 nm. To evaluate the effect of ZnO NPs on seed germination, various concentrations of nanoparticles (20, 50 and 100 ppm) were used for the treatment of wheat seed but there was not observed any significant effect on germination percentage, speed of germination and mean germination time. However, root length, shoot length, seedling length, root dry weight, shoot dry weight, seedling dry weight and seed vigour index (I and II) showed a significant increase at 50 ppm. Further, by increasing the concentration of ZnO NPs (at 100 ppm), a decrease was observed in all parameters which stipulated the toxicity of synthesized nanoparticles at higher concentration. Thus, the results of this study demonstrated that Catharanthus roseus leaf can be used as a good source for green synthesis of ZnO NPs and the treatment of ZnO NPs influenced the seedling growth and Seed vigour index significantly.


Keywords: Zinc Oxide Nanoparticles, Catharanthus roseus, Green Synthesis, Seed germination, Seedling growth

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How to cite this article:

Bhupendra Bhaskar Meher, Shivraj Sahu, Shivangi Singhal, Mayank Joshi, Poonam Maan and Sneh Gautam. 2020. Influence of Green Synthesized Zinc Oxide Nanoparticles on Seed Germination and Seedling Growth in Wheat (Triticum aestivum).Int.J.Curr.Microbiol.App.Sci. 9(5): 258-270. doi: https://doi.org/10.20546/ijcmas.2020.905.029
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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