National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Outer membrane vesicles (OMV) are closed spheroid vesicles with size ranging between 20–500 nm produced by both Gram negative and Gram positive bacteria. OMV are formed by the blebbing and pinching off segments of bacterial outer membrane. They are composed of lipopolysaccharide (LPS), glycerophospholipids, outer membrane proteins (OMPs), and periplasmic components. They are found in a variety of environments including liquid culture, solid culture and biofilms as well as during periods of bacterial stress. Being small size and having immunogenic properties and non-replicative behavior they stand as a good choice for vaccination. Keeping in view of the above facts the present study has been carried out to explore the immunogenic properties of OMV by its isolation and characterization. Isolation of OMV from Brucella abortus S19 was carried out by growing in solid as well as liquid media and subsequently the growth was subjected to filtration and centrifugation steps. The OMV were characterized by electron microscopy. They were further analyzed by SDS-PAGE and Western Blotting. On electrophorectic profile they revealed protein bands of 86 kDa, 70Kda, 65kDa 40 kDa and immunore active band of 86, 70, 65 kDa on western blotting.
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