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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 112-118
DOI: https://doi.org/10.20546/ijcmas.2020.905.011


Study of Outer Membrane Vesicles Isolated from B. abortus S19 by Bulk Production and Characterization
Losa Rose1*, Tejinder Singh Rai1, A. K. Arora1, Dipak Deka2 and L. Geeta Devi3
1Department of Veterinary Microbiology, 2College of Animal Biotechnology,
3Department of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Science University, Ludhiana, Punjab, 141004, India
*Corresponding author
Abstract:

Outer membrane vesicles (OMV) are closed spheroid vesicles with size ranging between 20–500 nm produced by both Gram negative and Gram positive bacteria. OMV are formed by the blebbing and pinching off segments of bacterial outer membrane. They are composed of lipopolysaccharide (LPS), glycerophospholipids, outer membrane proteins (OMPs), and periplasmic components. They are found in a variety of environments including liquid culture, solid culture and biofilms as well as during periods of bacterial stress. Being small size and having immunogenic properties and non-replicative behavior they stand as a good choice for vaccination. Keeping in view of the above facts the present study has been carried out to explore the immunogenic properties of OMV by its isolation and characterization. Isolation of OMV from Brucella abortus S19 was carried out by growing in solid as well as liquid media and subsequently the growth was subjected to filtration and centrifugation steps. The OMV were characterized by electron microscopy. They were further analyzed by SDS-PAGE and Western Blotting. On electrophorectic profile they revealed protein bands of 86 kDa, 70Kda, 65kDa 40 kDa and immunore active band of 86, 70, 65 kDa on western blotting.


Keywords: Outer membrane vesicles, Brucella abortus, Vaccine, SDS-PAGE, Western Blotting.
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How to cite this article:

Losa Rose, Tejinder Singh Rai, A. K. Arora, Dipak Deka, L. Geeta Devi. 2020. Study of Outer Membrane Vesicles Isolated from B. abortus S19 by Bulk Production and Characterization.Int.J.Curr.Microbiol.App.Sci. 9(5): 112-118. doi: https://doi.org/10.20546/ijcmas.2020.905.011