Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 2564-2575
DOI: https://doi.org/10.20546/ijcmas.2020.904.308


Morphological and Molecular Characterization of Gastrointestinal Nematodes in Sheep
Veena1*, G. C. Puttalakshmamm1, K. J. Ananda2 and P. P. Sengupta3
KVAFSU, Department of Parasitology, Veterinary College, Hassan-573202, Karnataka, India
*Corresponding author
Abstract:

A study was set out to investigate the distribution of gastrointestinal nematode parasites in sheep farms of Hassan district, Karnataka and to ascertain which species are involved in the anthelmintic resistance. In the study 26 farms were treated with fenbendazole and 19 farms were treated with ivermectin. The pre and post treatment faecal cultures were prepared for each farms. The larvae identified in the pre-treatment faecal culture were Haemonchus sp, Trichostrongylus sp, Oesophagostomum sp, Bunostomum sp and Cooperia sp based on the morphological keys. During the study 15 farms showed resistance to fenbendazole whereas 2 farms were resistant to ivermectin. Among 15 farms, 12 farms had Haemonchus sp as a predominant larvae followed by Trichostrongylus sp in 3 farms. The ivermectin resistant farms had Haemonchus sp as pre dominant larvae. The post treatment faecal cultures revealed Haemonchus sp. as the predominant gastrointestinal nematode followed by Trichostrongylus sp. The ones mentioned were identified as Haemonchus contortus and T. colubriformis by PCR. The PCR tool used for epidemiological studies supported the investigations of conventional parasitological techniques.


Keywords: Haemonchus contortus, Trichostrongylus colubriformis,PCR, Anthelmintic resistance, ITS-2
Download this article as Download

How to cite this article:

Veena, M., G. C. Puttalakshmamm, K. J. Ananda and Sengupta, P. P. 2020. Morphological and Molecular Characterization of Gastrointestinal Nematodes in Sheep.Int.J.Curr.Microbiol.App.Sci. 9(4): 2564-2575. doi: https://doi.org/10.20546/ijcmas.2020.904.308