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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 2474-2487
DOI: https://doi.org/10.20546/ijcmas.2020.904.296


Isolation, Morphometrics and Morphological Characterization of Oscheius chongmingensis from Assam, India
Bharath Amuri and Gitanjali Devi
Department of Nematology, Assam Agricultural University, Jorhat, Assam, India
*Corresponding author
Abstract:

A total 200 soil samples were collected from tea plantation areas of district, Jorhat, Assam and were assessed for entomopathogenic nematodes using the Galleria baiting technique. Out of 200 soil samples, EPNs were found in 1 soil samples collected from Experimental farm for plantation crops, Section-4,10,19AAU, Jorhat with 0.5% frequency of occurrence. The measurements were expressed in % ratios and Means ± SD ranges. The IJs of EPN-O-J-1 isolate were characterized by the position of excretory pore (97 vs. 90), lower tail length (87 vs. 111), higher E% (111vs. 83) and ratio c (5.22 vs. 3.9). The male of this second-generation body is curved ventrally like J shaped when heat-killed; lower body length (1042 vs. 1115), higher body width (54.8vs. 46) and GS (48.8vs.48) and the first-generation hermaphroditic female of this isolate body was curved ventrally when heat-killed and measured about 1742±288µm(1362-2340µm) in body length, 98.2±18.4µm(77-138µm) in body width, 109.6±18.0µm(83-136µm) in tail length, 27.5±4.4µm(21-39µm) in anal body width and 50.11±4.06µm(43.2-55.4µm) in V%, respectively. The The second generation amphimictic females of isolate EPN-O-J-l were also showed ventrally curved body when heat killed and measured about 1085.7±174.9µm(753-1392µm) in body length, 55.1±10.1µm(34-76µm) in body width, 98.5±21.8µm(72-158µm)in tail length, 28±5.5µm(23-42.2µm) and 52.72±3.69µm(48-62µm) in V%. Comparative analysis revealed that an EPN-O-J-l isolates belongs to the Oscheius chongmingensis as earlier described by Zhang et al., 2008from Chongming Island in eastern China in respect of body length, body width, tail length, higher E%, GS, ratio c and V%, respectively.


Keywords: Oscheius chongmingensis, Tea and Galleria
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How to cite this article:

Bharath Amuri and Gitanjali Devi. 2020. Isolation, Morphometrics and Morphological Characterization of Oscheius chongmingensis from Assam, India.Int.J.Curr.Microbiol.App.Sci. 9(4): 2474-2487. doi: https://doi.org/10.20546/ijcmas.2020.904.296