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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 1045-1054
DOI: https://doi.org/10.20546/ijcmas.2020.904.124


Analysis of Penton Base Gene of Fowl Adenoviruses Reveals Recombination among Different Species of Aviadenovirus Genus
Vipan Kumar1*, Rajesh Chandra2, Rinmuanpuii Ralte1 and Sarnarinder Singh Randhawa1
1Department of Veterinary Microbiology, Khalsa College of Veterinary and Animal Sciences, Amritsar-143001, India
2Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar-263145, India
*Corresponding author
Abstract:

The fowl adenoviruses have been studied extensively because of their involvement in many pathogenic conditions of poultry and considerable interest to use as gene delivery vehicles. The major structural proteins of virion include hexons and pentons (penton base and fiber). Among these proteins, only hexons have been characterized extensively at molecular level. The present study was conducted for molecular characterization of penton base gene. Liver homogenates of nine fowl adenovirus 4 (FAdV 4) field isolates were propagated in chicken embryo liver cell culture and total genomic DNA was extracted. The PCR amplification revealed a 1675 bp product. Further cloning was done in pGEM-T vectors and subjected to sequencing. The nucleotide sequences were compared with other sequences of NCBI database. The sequence homology within FAdV 4 isolate sequences was 99% to 100%, with FAdV10 99% , with FAdV A 67% and FAdV D 69-70 %. Phenogram analysis of nucleotide sequences showed close association between FAdV 4 and FAdV 10 but amino acid sequences revealed differences and recombinations were evident. Primary structural analysis revealed highly fragile nature of penton base protein. Secondary structure analysis revealed high concentration of proline residues at N-terminal ends signifying its role in virus-host interactions and bonding with other structural proteins.


Keywords: Fowl adenovirus (FAdV), Inclusion Body Hepatitis (IBH), Hydropericardium-hepatitis syndrome (HHS), Penton base gene, Phenogram analysis
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How to cite this article:

Vipan Kumar, Rajesh Chandra, Rinmuanpuii Ralte and Sarnarinder Singh Randhawa. 2020. Analysis of Penton Base Gene of Fowl Adenoviruses Reveals Recombination Among Different Species of Aviadenovirus Genus.Int.J.Curr.Microbiol.App.Sci. 9(4): 1045-1054. doi: https://doi.org/10.20546/ijcmas.2020.904.124