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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 249-258
DOI: https://doi.org/10.20546/ijcmas.2020.904.030


Bio-Molecular Studies in NaCl Induced Vegetatively Propagated
Excoecaria agallocha L. during Hardening
Pradeep Kumar Maharana* and Uday Chand Basak
Department of Seed Bank and Seed Biology Division, Regional Plant Resource Centre, Bhubaneswar-751015, India
*Corresponding author
Abstract:

Effects of salinity (NaCl) on protein content and antioxidant activity of vegetatively propagated plantlets of Excoecaria agallocha were evaluated during hardening period. The vegetatively propagated E. agallocha plantlets were treated with different concentration of salinity i.e. 0, 100, 200, 300, 400 and 500 mM NaCl for 28 days where bio-molecular parameters were observed at zero, 7th, 14th, 21st and 28th day of NaCl treatments. Three distinct and thick protein/polypeptide bands at 43, 20.1 and 14.3 kDa were detected in both with or without salt treated saplings during qualitative analysis of E. agallocha leaf protein. This indicates E. agallocha can survive wide salinity range. The lowest value of IC50 value in 200mM NaCl (at 21st day) and highest value of total phenolic content in 300mM (at 14th day), total flavonoid content in 200mMNaCl (at 28thday), reducing power in 200mM NaCl (at 21stday), proline content in 300 mM NaCl (at 14th day) and glycine betaine content in 400mM NaCl (at 14th day) could be the biochemical marker. This research work can provide preliminary data on salt tolerant behavior of vegetatively propagated E. agallocha plantlets during hardening period; which can facilitate further course of research on its field transfer.


Keywords: Excoecaria agallocha, Vegetative propagation, NaCl, Protein, Antioxidant activity
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How to cite this article:

Pradeep Kumar Maharana and Uday Chand Basak. 2020. Bio-Molecular Studies in NaCl Induced Vegetatively Propagated Excoecaria agallocha L. during Hardening.Int.J.Curr.Microbiol.App.Sci. 9(4): 249-258. doi: https://doi.org/10.20546/ijcmas.2020.904.030