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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 33-42
DOI: https://doi.org/10.20546/ijcmas.2020.904.006


Random Amplified Polymorphic DNA (RAPD) Analysis of Human papilloma virus Isolated from Cervical Cancer Patient
Ayu Novita Trisnawati1, Annisa Fatharani2, Florence Clay Mora2, Yufri Aldi2,Andani Eka Putra3, Yoke Kqueen Cheah4 and Marlina2*
1Department of Biotechnology, Post Graduate Program, Andalas University, Padang,
West Sumatera, Indonesia
2Faculty of Pharmacy, Andalas University, Padang, West Sumatera, Indonesia
3Departement of Microbiology, Faculty of Medicine, Andalas University, Padang,
West Sumatera, Indonesia
4Department of Biomedical Science, Faculty of Medicine and Health Sciences,
Universiti Putra Malaysia, Selangor, Malaysia
*Corresponding author
Abstract:

Cervical cancer prevalence is increasing every year. The major cause of this cervical cancer is the infection of high-risk Human papillomavirus (HPV) types. HPV variation is related to its ethnicity and geographical source. The objective of this study is to analyze HPV genetic diversity from the two Sumatran regions, Pekanbaru and Padang. Twenty DNA samples from cervical cancer patients were used in this study. HPV detection was carried out using MY09/11 primer. Positive HPV samples were amplified with OPO-6, OPO-18 and OPO-19 primer, and the dendrogram was constructed with UPGMA (Unweighted Pair Group Method with Arithmetic Mean) method. HPV was detected in fourteen DNA samples. PCR amplification by OPO primer showed multiple bands. The dendrogram results of the three primers, DNA samples divided into two groups X and Y. Based on the dendrogram, HPV classified on genotypes and source origin. RAPD data describe HPV variance, but still need further analysis for comprehensive HPV diversity.


Keywords: Genetic relationship, Diversity, PCR, Dendrogram, HPV 16, HPV 18
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How to cite this article:

AyuNovita Trisnawati, Annisa Fatharani, Florence Clay Mora, Yufri Aldi, AndaniEka Putra, Yoke Kqueen Cheah and Marlina. 2020. Random Amplified Polymorphic DNA (RAPD) Analysis of Human papilloma virus Isolated from Cervical Cancer Patient.Int.J.Curr.Microbiol.App.Sci. 9(4): 33-42. doi: https://doi.org/10.20546/ijcmas.2020.904.006