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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 3063-3071
DOI: https://doi.org/10.20546/ijcmas.2020.903.351


Effect of Supplementation of Glutathione and α-Tocopherol in Tris and Skim Milk Based Extenders on Motility Parameters of Ram Semen at Refrigeration Temperature
Sangamesh Muniyappanavar1*, M. K. Tandle2, P. T. Vinay2, R. G. Bijurkar2, M. D. Suranagi2, Shrikant Kulkarni2 and B. Bhagavantappa2
1Veterinary Officer, Belagavi, Karnataka, India
2KVAFSU, Bidar, Karnataka, India
*Corresponding author
Abstract:

The present study was conducted to evaluate the effect of supplementation of glutathione and α-tocopherol in TRIS and skim milk based extenders on motility parameters of ram semen at refrigeration temperature. The research findings of the present study shown that, addition of glutathione improved motile sperm and reduced immotile sperm percentage than α-tocopherol in skim milk than TRIS egg yolk based extenders. Addition of glutathione improved rapid progressive sperm percentage than α-tocopherol in TRIS egg yolk than skim milk based extenders. Addition of α-tocopherol in TRIS egg yolk reduced slow progressive sperm percentage, whereas improved in skim milk based extenders as compared to addition of glutathione. Addition of either glutathione or α-tocopherol did not reduce the non-progressive sperm percentage in skim milk and TRIS egg yolk based extenders. The volume of semen (Mean±SE) ranged from 0.83±0.04 to 0.94±0.03 mL having creamy colour. The mass activity of semen samples was with ++++ for all semen samples in NARI Suwarna strain of sheep. The addition of glutathione in skim milk based extender improved motile sperm percentage than TRIS egg yolk extender at 72 h of refrigeration. The addition of glutathione increased rapid progressive sperm percentage at 72 h of refrigeration in TRIS egg yolk than skim milk based extender.


Keywords: α-Tocopherol, Extenders, Motility parameters, TRIS egg yolk
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How to cite this article:

Sangamesh Muniyappanavar, M. K. Tandle, P. T. Vinay, R. G. Bijurkar, M. D. Suranagi, Shrikant Kulkarni and Bhagavantappa, B. 2020. Effect of Supplementation of Glutathione and α-Tocopherol in Tris and Skim Milk Based Extenders on Motility Parameters of Ram Semen at Refrigeration Temperature.Int.J.Curr.Microbiol.App.Sci. 9(3): 3063-3071. doi: https://doi.org/10.20546/ijcmas.2020.903.351