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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 2844-2856
DOI: https://doi.org/10.20546/ijcmas.2020.903.328


A Study to Comparison of MIC of Linezolid on MRSA by Micro Broth Dilution and E Strip Method in Teaching Hospital, Karnataka, India
R.Sharleeand B. Sumangala2*
1Department of Microbiology, Chamarajnagar Institute Medical Sciences, Chamarajanagar, Karnataka, India
2Department of Microbiology, Mandya Institute Medical Sciences,Mandya, Karnataka, India
*Corresponding author
Abstract:

Although for severe MRSA infections, vancomycin is described as the first-line intravenous drug, vancomycin-resistant and intermediate isolates of Staphylococcus aureus (VRSA & VISA) have been increasingly reported throughout the world. The therapeutic and life-saving option for VRSA and VISA infections is linezolid. Objective of our study was to assess Minimum inhibitory concentration of linezolid on MRSA by micro broth dilution and E strip method. One hundred fifty isolates from clinical samples were processed. Colonies suggestive of Staphylococcus aureus were identified as MRSA using Cefoxitin (30µg) discasper Clinical and Laboratory Standards Institute (CLSI) guidelines. MRSA Positive isolates were tested for Minimum inhibitory concentration of linezolid by E Strip and Micro broth dilution method. In our study out of 150 isolates of Staphylococcus aureus, 65(43.3%) were MRSA and 85(56.7%) were MSSA.   A total of 65 MRSA isolates were tested for MIC by Estrip and Micro broth dilution, which showed 100% sensitivity for linezolid. In the present study, did not come across any resistant strain. In the study total of 65 isolates were tested, According to MIC of linezolid by E Strip which majority (36.9%) had MIC <2μg/ml followed by 30.8% with MIC<1μg/ml, 23% with MIC <4μg/ml and 9.2% with MIC <0.5μg/ml. In comparison to our study  approximately similar MIC values were noted in the study conducted by Stefan Riedel et al.,  showing  65.1% with MIC <1μg/ml, 27.9% with MIC <2μg/ml, 4.7% with MIC <0.5μg/ml  and 2.3% with MIC <4μg/ml.According to MIC of linezolid by micro broth dilution  majority 63.1% of the isolates were having MIC <1μg/ml, followed by 26.1% with MIC <2μg/ml, 6.1% with MIC <4μg/ml  and 4.7% with MIC <0.5μg/ml.


Keywords: MRSA, MIC, E-strips, Micro broth dilution, linezolid
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How to cite this article:

Sharlee. R and Sumangala. B. 2020. A Study to Comparison of MIC of Linezolid on MRSA by Micro Broth Dilution and E Strip Method in Teaching Hospital, Karnataka.Int.J.Curr.Microbiol.App.Sci. 9(3): 2844-2856. doi: https://doi.org/10.20546/ijcmas.2020.903.328