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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 594-602
DOI: https://doi.org/10.20546/ijcmas.2020.903.070


Prevalence and Characterization of Extended Spectrum Beta Lactamase Producing Escherichia coli from Broilers
Susmita Pal1, Samir Dey1*, Kunal Batabyal1, Abhiroop Banerjee2,
Siddhartha Narayan Joardar1, Indranil Samanta1 and Devi Prasad Isore1
1Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences (WBUAFS), Belgachia, Kolkata-700037, India
2West Bengal University of Animal and Fishery Sciences, Kolkata-700037, India
*Corresponding author
Abstract:

In the current study prevalence and characterization of extended spectrum beta-lactamase(ESBL) production of Escherichia coli was detected from broilers of West Bengal, India. A total of 248 cloacal swabs samples were examined for E.coli by standard bacteriological techniques. E.coli isolates were serotyped and also screened for extended spectrum beta-lactamase by double disc diffusion assay and by PCR detection of blaCTX-M, blaSHV, and blaTEM genes. The antimicrobial sensitivity profile of the isolated E.coli was demonstrated against 13 antimicrobial agents. One hundred thirty one (52.82%) E.coli were identified and the isolates belonged to 12 different serogroups viz. O2, O20, O35, O83, O87, O88, O116, O117, O119, O128, O135, O141 and untyped isolates. Thirty seven (28.24%) of E.coli were detected as ESBL-positive by double disc diffusion assay. Among the studied genes, blaCTX-M gene was detected in 32 (24.43%), blaSHV gene in 3 (2.29 %) isolates and blaTEM gene in 2 (1.53%) isolates. Highest antimicrobial susceptibility of E.coli isolates was observed to chloramphenicol (69.36 %) and levofloxacin (62.9 %). Highest degree of resistance was observed to cefepime (95.12%) followed by nalidixic acid (88.88%), co-trimoxazole (83.33%), ampicillin (79.03%), cefotaxime (78.46%), tetracycline (72.58%) and ciprofloxacin (62.90%). The present study revealed that broilers were reserviors of ESBL producing E.coli and resistant to many classes of antimicrobials.


Keywords: Antimicrobial sensitivity, Broilers, disc, E.coli, ESBL, PCR
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How to cite this article:

Susmita Pal, Samir Dey, Kunal Batabyal, Abhiroop Banerjee, Siddhartha Narayan Joardar, Indranil Samanta and Devi Prasad Isore. 2020. Prevalence and Characterization of Extended Spectrum Beta Lactamase Producing Escherichia coli from Broilers.Int.J.Curr.Microbiol.App.Sci. 9(3): 594-602. doi: https://doi.org/10.20546/ijcmas.2020.903.070