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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 317-323
DOI: https://doi.org/10.20546/ijcmas.2017.603.035


A Simple Method for Transient Expression of Reporter Gene in Brinjal Leaves through Agroinfiltration
Vikash Kumar, Pawan Kumar and Tirthartha Chattopadhyay*
Department of Plant Breeding and Genetics, Bihar Agricultural College, Bihar Agricultural University, Sabour, Bhagalpur, Bihar, India-813210
*Corresponding author
Abstract:

Brinjal (Solanum melongena L.) is one of the most important vegetable crops in India. In spite of the docility of brinjal towards genetic transformation through Agrobacterium, development of stable transgenic lines in brinjal is a cost, labour as well as time consuming process. On the other hand, the agro infiltration process has been optimized in different crops for the rapid analysis of transgene through transient expression. The process has also gained importance in synthetic biological applications for rapid production of therapeutic biomolecules in plants. Here, we have evaluated the agroinfiltration process in brinjal leaves through transient expression of a reporter gene driven by a constitutive promoter. Following optimization of the incubation period, proper infiltration medium and the optical density of the Agrobacterium suspension, we advocate the usefulness of the process for gene function analysis and other possible downstream applications through transient expression of a transgene in brinjal.


Keywords: Agroinfiltration, Brinjal, Histochemical assay, Reporter gene, Transient expression.
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How to cite this article:

Vikash Kumar, Pawan Kumar and Tirthartha Chattopadhyay. 2017. A Simple Method for Transient Expression of Reporter Gene in Brinjal Leaves through AgroinfiltrationInt.J.Curr.Microbiol.App.Sci. 6(3): 317-323. doi: https://doi.org/10.20546/ijcmas.2017.603.035