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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 75-79
DOI: https://doi.org/10.20546/ijcmas.2020.903.009


First Molecular Detection of Anaplasma platys and Coinfection with Babesia gibsoni in Dogs from Bengaluru, India
Medha Karnik*, Anjan Kumar, M. Manjula, H. D. Lohitha, R. Narendra,K. Ashwin, R. K. Yashas, Rashmi Rajashekaraiah, H. R. Azeemullah, S. Ravi Kumar, G.K. Chetankumar, Mohan Kumar Shettar, P. T. Ramesh, N. Prakash, Ansar Kamran, Nitish Kalyanpur, Suguna Rao and M. L. Satyanaryana
1Department of Veterinary pathology, Veterinary college, Bengaluru-560024, India
2Division of Molecular Diagnostics, Vetlesions Veterinary Diagnostic Laboratory, Bengaluru, India
3Maruti Pet care Centre, Bengaluru, India
4Bangalore Pet Hospital, Bengaluru, India
5Cancure Pet Hospital, Bengaluru, India
6Neha Veterinary Cllinic, India
7Mypets choice Veterinary Clinic, India
*Corresponding author
Abstract:

Anaplasmosis, a tick-borne disease in dogs, caused by obligate intracellular pathogens, Anaplasma platys and Anaplasma phagocytophilum. Anaplasmosis is characterized by a variety of clinical symptoms including pyrexia as well as non-symptomatic thrombocytopenia. Advances in molecular techniques have enabled physicians and diagnosticians to use polymerase chain reaction for identification of theses pathogens in dogs. A total of 80 samples collected from the veterinary clinics in Bengaluru were considered for the study for screening Anaplasma platys and Anaplasma phagocytophilum by polymerase chain reaction. Seventeen out of eighty samples were positive for A. platys. Co-infection of A. platys with Babesia gibsoni and Ehrlichia canis was also reported in this study. Anaplasma platys positive samples were confirmed by sequencing and submitted to GenBank. No samples were found positive for Anaplasma phagocytophilum in the current study.


Keywords: Anaplasmosis, Anaplasma platys, 16srRNA, Co-infection, Sequencing, Polymerase chain reaction
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How to cite this article:

Medha Karnik, Anjan Kumar, M. Manjula, H. D. Lohitha, R. Narendra, K. Ashwin, R. K. Yashas, Rashmi Rajashekaraiah, H. R. Azeemullah, S. Ravi Kumar, G. K. Chetankumar, Mohan Kumar Shettar, P. T. Ramesh, N. Prakash, Ansar Kamran, Nitish Kalyanpur, Suguna Rao and Satyanaryana, M. L. 2020. First Molecular Detection of Anaplasma platys and Coinfection with Babesia gibsoni in Dogs from Bengaluru, India.Int.J.Curr.Microbiol.App.Sci. 9(3): 75-79. doi: https://doi.org/10.20546/ijcmas.2020.903.009