Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 2072-2084
DOI: https://doi.org/10.20546/ijcmas.2020.902.235


Comparison of Cleavage Rate of Buffalo Oocytes on Supplementing with Different Concentration of Serum Gonadotropin, Estradiol 17b, Estrus Buffalo Serum and Buffalo Follicular Fluid
Ambadas1*, K. Shrikant1, G. Sarvadnya2 and K. B. Satish1
1Department of Veterinary Physiology and Biochemistry, Veterinary College, Bidar, India
2Veterinary Officer, Veterinary Dispensary Donagapur Tq Bhalki Dist Bidar, India
*Corresponding author
Abstract:

Serum gonadotropin @50 and 100IU/ml, estrodiol-17β @0.5 and 1μg/ml, EBS @5 and 10% and BFF @5 and 10% supplementation Iis done on cleavage rate of buffalo oocytes for the comparison of efficacy of hormones. Ovaries were collected from buffaloes slaughtered at local abattoir. Oocytes were aspirated and good and excellent quality oocytes were matured in basic maturation media TCM199 supplementing above mentioned supplements separately. The recovery rate of culturable oocytes was 85.33%. The highest cleavage rate was documented with supplementation of 10% EBS and the lowest was observed with 10% BFF. The cleavage rate was significantly (p≤0.05) higher in the group supplemented with 5%EBS and 10% EBS as compared to all other groups of the experiment. It can be concluded that the supplementation of EBS to IVM medium has positive effect on cleavage rate.


Keywords: Cleavage rate, Oocytes, Serum gonadotropin, Follicular fluid
Download this article as Download

How to cite this article:

Ambadas, K. Shrikant, G. Sarvadnya and Satish, K. B. 2020. Comparison of Cleavage Rate of Buffalo Oocytes on Supplementing with Different Concentration of Serum Gonadotropin, Estradiol 17b, Estrus Buffalo Serum and Buffalo Follicular Fluid.Int.J.Curr.Microbiol.App.Sci. 9(2): 2072-2084. doi: https://doi.org/10.20546/ijcmas.2020.902.235