National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The irrational and indiscriminate use of antibiotics has made antibiotic resistance as biggest health problem of India. Therefore, the present study was undertaken with the aim to isolate ESBL producing E. coli and Klebsiella from clinical human cases. A total of 100 urine samples of human were collected from pathology lab of Faizabad and Gorakhpur district and processed for isolation of E. coli and Klebsiella. All isolates were screened for ESBLs production by using Cefotaxim and Ceftazidime disk. The presumptive ESBL producers were further confirmed by combination disk test using ESBL kits and by targeting ESBL genes using PCR technique. The isolation rate of E. coli and Klebsiella was found to be 24% and 26% and all were found presumptive ESBL producers on preliminary screening. Further confirmation by combination disk test revealed 41.7% and 61.5% prevalence of ESBLs in E. coli and Klebsiella. The molecular study revealed blaTEM gene in only one (10%) E. coli isolate, while blaCTX-M gene was prevalent in 70% E. coli isolates and 93.75% Klebsiella isolates. Conclusions: The present study showed predominance of blaCTX-M gene in both E. coli and Klebsiella which indicates irrational use of antibiotics in human medicine posing a great risk to the health of both human and animal population.
Citations |
 |
 |
 |
 |
