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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 357-364
DOI: https://doi.org/10.20546/ijcmas.2020.902.045


Isolation and Identification of Extended-Spectrum Beta-Lactamases Producing E. coli and Klebsiella from Human
Rajeev Prajapati1, Namita Joshi1* and R.K. Joshi2
1Department of Veterinary Public Health & Epidemiology, College of Veterinary Science & A.H., N.D. University of Agriculture and Technology, Kumarganj- 224229, Faizabad (UP), India
2Department of Veterinary Microbiology, College of Veterinary Science & A.H., N.D. University of Agriculture and Technology, Kumarganj- 224229, Faizabad (UP), India
*Corresponding author
Abstract:

The irrational and indiscriminate use of antibiotics has made antibiotic resistance as biggest health problem of India. Therefore, the present study was undertaken with the aim to isolate ESBL producing E. coli and Klebsiella from clinical human cases. A total of 100 urine samples of human were collected from pathology lab of Faizabad and Gorakhpur district and processed for isolation of E. coli and Klebsiella. All isolates were screened for ESBLs production by using Cefotaxim and Ceftazidime disk. The presumptive ESBL producers were further confirmed by combination disk test using ESBL kits and by targeting ESBL genes using PCR technique. The isolation rate of E. coli and Klebsiella was found to be 24% and 26% and all were found presumptive ESBL producers on preliminary screening. Further confirmation by combination disk test revealed 41.7% and 61.5% prevalence of ESBLs in E. coli and Klebsiella. The molecular study revealed blaTEM gene in only one (10%) E. coli isolate, while blaCTX-M gene was prevalent in 70% E. coli isolates and 93.75% Klebsiella isolates. Conclusions: The present study showed predominance of blaCTX-M gene in both E. coli and Klebsiella which indicates irrational use of antibiotics in human medicine posing a great risk to the health of both human and animal population.


Keywords: ESBL, Klebsiella, E. coli, urine, Haemagglutination
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How to cite this article:

Rajeev Prajapati, Namita Joshi and R.K. Joshi. 2020. Isolation and Identification of Extended-Spectrum Beta-Lactamases Producing E. coli and Klebsiella from Human.Int.J.Curr.Microbiol.App.Sci. 9(2): 357-364. doi: https://doi.org/10.20546/ijcmas.2020.902.045