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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 51-65
DOI: https://doi.org/10.20546/ijcmas.2020.902.008


CMS Line in Carrot
Chetna Chugh1*, Sheshnath Mishra2, Manisha Mangal3, Shrawan Singh3 and Pritam Kalia3
1Division of Biochemistry, ICAR-Indian Agricultural Research Institute,New Delhi-110012, India
2Shri Vaishnav Institute of Agriculture Science, Shri Vaishnav Vidyapeeth Vishwavidyalaya, Indore-453111, India
5Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
*Corresponding author
Abstract:

Male sterility in vegetables is commercially utilized to develop their hybrids due to cost effective and easy mechanism to produce large-scale hybrid seeds commercially. Different types of male sterility, and three-line and two-line hybrid breeding approaches involving Cytoplasmic male stesility (CMS) and environmental sensitive genic male sterile (GMS) lines, are used for hybrid seed production.  In carrot which produces many but small sized flowers, making hand-emasculation tedious and time consuming. Identification of male sterile and fertile counterpart of CMS lines is also not possible at early seedling stage by using test cross methods. Marker assisted selection for male sterile and fertile (maintainer) genes greatly enhance selection of A and B plants in breeding program. So, molecular markers that can distinguish CMS status and fertile plants at an early seedling stage will be valuable tool in carrot breeding including CMS based hybrid seed programs in hybrid seed purity evaluation, selection and management. Keeping these in views, the present study was undertaken to characterize different sources of cytoplasmic male sterile lines and their maintainers. By using available molecular markers closely linked to sterility gene to distinguish our male sterile and fertile lines.


Keywords: Hybrid carrot seed, Cytoplosmic male sterility, Maintainer, Molecular Markers, PCR.
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How to cite this article:

Chetna Chugh, Sheshnath Mishra, Manisha Mangal, Shrawan Singh, Pritam Kalia. 2020. CMS Line in Carrot.Int.J.Curr.Microbiol.App.Sci. 9(2): 51-65. doi: https://doi.org/10.20546/ijcmas.2020.902.008