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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 2387-2396
DOI: https://doi.org/10.20546/ijcmas.2020.901.272


Axenic Germination of Glomus intraradices in in vitro
K.S. Raghavendra*, P. Jones Nirmalnath and K.S. Jagadeesh
Department of Agricultural Microbiology, College of Agriculture,
University of Agricultural Sciences, Dharwad - 580 005, Karnataka, India
*Corresponding author
Abstract:

Arbuscular mycorrhizal (AM) fungi are obligate symbiont and integrate with most of terrestrial plants. AMF belongs to the phylum Glomeromycota that cannot complete their life cycle without establishing a functional symbiosis with host plant. In the present investigation, Glomus intraradices Spores were surface sterilized and transferred to different media viz., one per cent water medium agar medium, Murashige and Skoog medium. Modified Strulla Romand (MSR) medium and white’s medium with root exudates of Agrobacterium rhizogenes MTCC-532 transformed hairy roots of chickpea. Surface sterilized spores were spread on the per Petri plate with five replications for germination test. The petriplates were incubated at 240C under dark condition. Spore germination study revealed that germination and germ tube growth of G. intraradices was influenced by root exudates in all the medium. The results have clearly shown that the MSR medium incorporated with root exudates recorded the maximum spore germination (93.3 per cent) and the highest initiation of germ tube was recorded at 35 DAI (61.05 mm).


Keywords: Spore germination, Germ tube induction, Glomus intraradices, Root exudates.
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How to cite this article:

K.S. Raghavendra, P. Jones Nirmalnath and K.S. Jagadeesh. 2020. Axenic Germination of Glomus Intraradices in in vitroInt.J.Curr.Microbiol.App.Sci. 9(1): 2387-2396. doi: https://doi.org/10.20546/ijcmas.2020.901.272