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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 2299-2305
DOI: https://doi.org/10.20546/ijcmas.2020.901.261


Influence of different Extraction and Fractionation Periods on Humic Acid Yield derived from Pressmud and Vermicompost
Shreelatha1, S. N. Bhat1*, S. R. Balanagoudar1, R. V. Beladhadi1,
Satyanarayana Rao2 and M. V. Ravi1
1Department of Soil Science and Agriculture Chemistry, 2Department of Agronomy,University of Agricultural Sciences, Raichur- 584 104(Karnataka), India
*Corresponding author
Abstract:

Humic acids (HA) are widely used in agriculture because of their capability as chelator, organic matter, and soil improver and growth promoter. However, their isolation differs in terms of origin or source because of wetting and solubilisation factors. This study was conducted to determine extraction period for HA from organic sources. Standard procedures were used to extract HA from pressmud (PM) and vermicompost (VC) at different extraction periods of 4, 8, 12, 16, 20 and 24 hours and fractionation periods (3, 6, 9, 12, 15, 18, 21& 24 hrs). At the end of each extraction period, samples were centrifuged, followed by fractionation and purification. HA yield at different extraction periods was determined using standard procedures. The relationship between extraction period and HA yield was quadratic. Extraction and fraction period for PM & VC was 24 hours and purification (done in 1 h) using distilled water was effective in removing impurities. HA from PM and VC can be extracted and fractionated in 24 hours and purification can be done in one hour.


Keywords: Humic acid, Pressmud, Vermicompost, Extraction and fractionation period
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How to cite this article:

Shreelatha, S. N. Bhat, S. R. Balanagoudar, R. V. Beladhadi, Satyanarayana Rao and Ravi, M. V. 2020. Influence of different Extraction and Fractionation Periods on Humic Acid Yield derived from Pressmud and Vermicompost. Int.J.Curr.Microbiol.App.Sci. 9(1): 2299-2305. doi: https://doi.org/10.20546/ijcmas.2020.901.261