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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 2157-2169
DOI: https://doi.org/10.20546/ijcmas.2020.901.246


Molecular Characterization of Dendrobium Orchid Species from Western Ghat Region of Karnataka using RAPD and SSR Markers
Basavaraj1, N. Nagesha1* and M. Jadeyegowda2
1Department of Plant Biotechnology, College of Agriculture, University of Agricultural Sciences, GKVK, Bengaluru-560065, Karnataka, India
2Department of Natural Resource Management, College of Forestry, Ponnampet-571216 Karnataka, India
*Corresponding author
Abstract:

Orchids are the most beautiful flowering, highly evolved ornamental plants and also have medicinal importance.  In the present study, genetic diversity among six Dendrobium species was analysed using morphological characters and molecular markers including RAPD and SSR markers. Morphological characters analysed were leaf shoot and flower characters of six Dendrobium species. The variability in morphology was found among the species. Further, the genetic diversity among the species was determined by using RAPD and SSR markers. Out of 52 RAPD primers, 34 were selected for diversity analysis, producing 222 amplified bands. Of which 216 bands depicted 97.29 per cent polymorphism with Jaccard’s similarity coefficient varied from 0.19 to 0.35. The dendrogram was constructed by Unweighted Pair Group Method Using Arithmetic Average (UPGMA), separated six Dendrobium species into two main clusters, one with four species and the other with two species. Out of 11 SSR primers, eight were selected for diversity analysis, producing 12 amplified bands. Of which eight bands depicted 66.66 per cent polymorphism with Jaccard’s similarity coefficient varied from 0.37 to 0.60. The dendrogram was constructed by UPGMA, separated six Dendrobium species into two main clusters, one with five species and the other with one species. RAPD markers were more reliable than the SSR markers. Species specific SSR markers are to be developed in future.


Keywords: Dendrobium, Morphological characters, Genetic diversity, RAPD marker, SSR marker
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How to cite this article:

Basavaraj, B., N. Nagesha and Jadeyegowda, M. 2020. Molecular Characterization of Dendrobium Orchid Species from Western Ghat Region of Karnataka using RAPD and SSR Markers. Int.J.Curr.Microbiol.App.Sci. 9(1): 2157-2169. doi: https://doi.org/10.20546/ijcmas.2020.901.246