Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 1750-1759
DOI: https://doi.org/10.20546/ijcmas.2020.901.195


Prevalence of Non-Fermenting Gram Negative Bacilli from Clinical Isolates and their Antibiogram Profile
Rajeev Kumar1, Rachana Patel2*, Mannu Jain1 and Ankita Mistry1
1Department of Microbiology, SMIMER, Surat, Gujarat, India
2Department of Microbiology, Parul Institute of Medical Sciences and Research,Vadodara, Gujarat, India
*Corresponding author
Abstract:

NFGNB also known as Non-fermenters (NFs) are emerging with increasing frequency as agents of opportunistic and often, serious infection as well as nosocomial infection. NFGNB are innately resistant to many antibiotics and are known to produce extended spectrum β-lactamases and metallo β-lactamases (MBL).Very few laboratories in India identify these organisms as routine as they are slow growing and requires special media and biochemical tests for identification. The rates of isolation of NFGNBs are increasing in Clinical Microbiology Laboratory, hence this study was done. To identify the Non –fermenters isolated from various clinical samples, to determine the antibiotic resistance pattern and To test the metallo β- lactamase activity of the isolated Non – fermenters. In This study comprises of 2758 samples of clinical specimen among 192 non-fermenting gram negative bacilli isolated from various clinical specimens like Respiratory tract samples, pus wound swab, urine and blood samples are taken as standard conventional methods. Non fermentative Gram negative bacilli (NFGNB) up to genus or species level were identified and processed by standard conventional method. Antibiotic susceptibility pattern of isolates was studied by Kirby Bauer Disc diffusion technique and resistant pattern also found. A total of 192 clinically significant culture isolates were obtained from different clinical samples using standard conventional methods. Pseudomonas aeruginosa was most common isolate (56.77%), followed by Acinetobacter baumanii (36.97 %),  A.lwoffii(2.08%), P. fluorescens (4.16 %). Out of 49 Imipenem resistant organisms 22(44.89%) were Metalloβlactamase positive & 27 (55.10%) were Metalloβlactamase negative. They highly sensitive to Polymixin B and variable to carbapenamase group. The major risk factors for infection with non-fermenting gram negative bacilli infection were hospitalization of 5 day or more, surgical intervention and catheterization.


Keywords: Non-Fermenting Gram Negative Bacilli, β- lactamase activity, Pseudomonas aeruginosa
Download this article as Download

How to cite this article:

Rajeev Kumar, Rachana Patel, Mannu Jain and Ankita Mistry. 2020. Prevalence of Non-Fermenting Gram Negative Bacilli from Clinical Isolates and their Antibiogram Profile.Int.J.Curr.Microbiol.App.Sci. 9(1): 1750-1759. doi: https://doi.org/10.20546/ijcmas.2020.901.195