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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 1730-1735
DOI: https://doi.org/10.20546/ijcmas.2020.901.192


Expression of RelA, IKBα and IKKβ Genes in peste des petits
Ruminants Virus Infected Cells
Siddharth Gautam1*, Amol Gurav1, Muthu Sankar1, Nitish S. Kharayat1, A. P. Madhusoodan1, A. Rialch1, D. Muthuchelvan2, Chitra Joshi3 and Anil K. Sharma4
1Division of Temperate Animal Husbandry; 2Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteshwar, Nainital, Uttarakhand, India
3Animal Husbandry Department, Uttarakhand, India
4Division of Pathology, ICAR- Indian Veterinary Research Institute, Izatnagar,Bareilly, U.P., India
*Corresponding author
Abstract:

Peste des petits ruminants (PPRV) is a highly contagious transboundary viral disease of small ruminants with widespread distribution and poorly characterized molecular pathogenesis. The present study aimed at delineating the role of NF-kB signalling pathway during PPRV infection in the in-vitro system. For this the gene expression of three major regulatory proteins of NF-kB pathway viz. IKBα, IKKβ and RelA were analysed in the PPRV infected and mock infected Vero cells  as a function of time using SYBR-green based one-step RT-qPCR. The results genes revealed downregulation of IKBα gene and upregulation of RelA and IKKβ genes in the PPRV infected group as against mock infected during initial hours of infection.


Keywords: RelA, IKBα and IKKβ, peste des petits, RT-qPCR
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How to cite this article:

Siddharth Gautam, Amol Gurav, Muthu Sankar, Nitish S. Kharayat, A. P. Madhusoodan, A. Rialch, D. Muthuchelvan, Chitra Joshi and Anil K. Sharma. 2020. Expression of RelA, IKBα and IKKβ Genes in peste des petits Ruminants Virus Infected Cells.Int.J.Curr.Microbiol.App.Sci. 9(1): 1730-1735. doi: https://doi.org/10.20546/ijcmas.2020.901.192