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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 1327-1338
DOI: https://doi.org/10.20546/ijcmas.2020.901.147


Detection of Variousβ-Lactamases and their Co-production by Novel Disc Placement method among Gram Negative Bacilli isolates from patients with Spontaneous Bacterial Peritonitis
Kunal Bansal1, Ritu Aggarwal2*, H K Aggarwal3, Aparna Yadav4,
Priyanka Yadav1 and Priyanka Bansal5
1HCMS-I,Rohtak, Haryana, India
2Department of Microbiology, Rohtak, Haryana, India
3Department of Medicine, Rohtak, Haryana, India
4Department of Microbiology, Rohtak, Haryana, India
5Department of Anaesthesiology & Critical care, Rohtak, Haryana, India
PGIMS, Rohtak, Haryana, India
*Corresponding author
Abstract:

Gram-negative bacilli (GNB) are most frequently isolated bacteria from ascitic fluid aspirate of patients with Spontaneous bacterial peritonitis (SBP). Aetiology of SBP has been changing in recent years with increased involvement of multidrug resistant (MDR) bacteria as a result of production of various β-lactamases. The present study was done for detection of different β-lactamases and their co-existence by noveldisc placement method among GNB isolates from patients with SBP. Disc panel containing ceftazidime (CA), ceftazidime/tazobactam (CA/TZ), cefepime (CFP), ceftazidime/ tazobactam/ cloxacillin (CA/TZ/CLOX), imipenem (IMP) and Mercaptopropionic acid (MPA) was used. Pureextended spectrum β-lactamase (ESBL) production was found in 18 of 42 isolates (42.86%). Maximum ESBL positive isolates were found in E.coli (13 out of 28 isolates).Pure AmpC production was found in 6 of 42 isolates (14.28%).Metallo-β-lactamases (MBL) production was found in only 2 of 42 isolates (4.76%). Co-production of ESBL+ AmpC was seen in 3 of 42 isolates (7.145). All ESBL positive isolates showed multidrug resistance pattern. Detection of β-lactamase producing isolates will provide knowledge regarding resistance pattern of bacterial strains in a particular geographical area. This will further help promote appropriate and judicious use of antibiotics.


Keywords: SBP, MDR, β-lactamases, ESBL, AmpC, MBL,Novel disc panel.
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How to cite this article:

Kunal Bansal, Ritu Aggarwal, H K Aggarwal, Aparna Yadav, Priyanka Yadav, Priyanka Bansal. 2020. Detection of Variousβ-Lactamases and their Co-production by Novel Disc Placement method among Gram Negative Bacilli isolates from patients with Spontaneous Bacterial Peritonitis. Int.J.Curr.Microbiol.App.Sci. 9(1): 1327-1338. doi: https://doi.org/10.20546/ijcmas.2020.901.147