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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 1299-1305
DOI: https://doi.org/10.20546/ijcmas.2020.901.143


Green Synthesis of Chitosan Based Copper Nanoparticles and their Bio-Efficacy against Bacterial Blight of Pomegranate (Xanthomonas axonopodis pv. punicae)
Chidanandappa1* and V. B. Nargund2
1Department of Plant Pathology, College of Agriculture, Dharwad, India
2University of Agricultural Sciences, Dharwad-580 005, Karnataka, India
*Corresponding author
Abstract:

A great interest towards investigating bactericidal properties of nanoparticles as an alternative to antibiotics has been initiated. Chitosan based nanoparticles (Ch-CuNPs) were prepared by use of  water soluble chitosan solution (1.0 %) with CuSO4.5H2O (10 mM) and pomegranate aril extract (1.4 %) under magnetic stirrer for 24 h at room temperature. The synthesized nanoparticles were characterized by Atomic Force Microscope (AFM) and Particle Size Analyzer (PSA) and tested for their antibacterial activity against pomegranate blight pathogen, Xanthomonas axonopodis pv. punicae. The average size of Ch-CuNPs was 89.39 nm. The synthesized nanoparticles were evaluated against Xap with eight concentrations. The results revealed that, chitosan-CuNPs were effectively inhibited the growth of Xap at 1000 ppm concentration  and remained on par with standard antibiotic check streptocycline of 500 ppm.


Keywords: Chitosan-Copper nanoparticles, Xanthomonas axonopodis pv. punicae,  pomegranate aril extract and chitosan.
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How to cite this article:

Chidanandappa and Nargund, V. B. 2020. Green Synthesis of Chitosan Based Copper Nanoparticles and their Bio-efficacy against Bacterial Blight of Pomegranate (Xanthomonas axonopodis pv. punicae). Int.J.Curr.Microbiol.App.Sci. 9(1): 1299-1305. doi: https://doi.org/10.20546/ijcmas.2020.901.143