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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 900-909
DOI: https://doi.org/10.20546/ijcmas.2020.901.100


Purification of Industrially Important Proteases from Pseudomonas Strain and Its Kinetic Studies
Mohinder Kaur1, Sheetal Rana2* and Kumari Manorma3
1Department of Basic Science (Microbiology), Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan-173230 (HP), India
2Department of organic agriculture and Natural Farming,
CSKHPKV, Palampur-176061 India
3Department of Basic Science (Microbiology), Dr. Yashwant Singh Parmar University of   Horticulture and Forestry, Nauni, Solan-173230 (HP), India
*Corresponding author
Abstract:

The aim of study was development of industrially and agriculturally important bacterial strains which produces antimicrobial protease. It is a kind of study that tends to contribute the potential Pseudomonas strains that may be helpful as plant growth promoting, biocontrol agent and may be used for the production of antimicrobial protease at industrial level. In the present study we have tried to isolate the fluorescent Pseudomonas sp. from three medicinal plants i.e., Achyranthus aspera, Elaegnus umbellate and Heracleum candicans. Three strains were isolated i.e., A4, H5 and E6, which were showed the high production of antibacterial, antifungal and proteolytic activity. The enzyme protease was purified from the strain H5 by using Sephadex G-75 column chromatography. Kinetic study of the purified protease was done by checking the effect of pH and temperature, substrate concentration, enzyme concentration and reaction time. Purified proteases can be exploited for industrial use as it is in high demands in different industries.


Keywords: Pseudomonas Antimicrobial, Protease, Purification, Antifungal activity
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How to cite this article:

Mohinder Kaur, Sheetal Rana and Kumari Manorma. 2020. Purification of Industrially Important Proteases from Pseudomonas Strain and Its Kinetic Studies.Int.J.Curr.Microbiol.App.Sci. 9(1): 900-909. doi: https://doi.org/10.20546/ijcmas.2020.901.100