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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 1465-1505
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.167


Phenotypic Detection and Genotypic Characterization of Metallo-Beta-Lactamases in Gram Negative Bacilli isolated from Mansoura University Hospital in Egypt
Maysaa El-Sayed Zaki, Mervat Mashaly* and Reham El Agamy
Clinical Pathology Department, Faculty of Medicine, Mansoura University-Egypt
*Corresponding author
Abstract:

Metallo-β-lactamases (MβLs) production in gram negative bacilli is an emerging health problem worldwide. We aimed to determine the occurrence of MβLs among gram negative bacilli (GNB) by phenotypic and genotypic methods in Mansoura University hospital, Egypt. We conducted a prospective study on 107 GNB isolates recovered from different clinical specimens collected from different patients. Species identification and antibiotic susceptibility profile was determined by MicroScan Wa1kAway system. GNB which showed resistance to imipenem and/or meropenem were screened for MBL production by double-disk synergy test (DDST). Phenotypically detected MBLs isolates were further studied for the presence of three genes; IMP, GIM and VIM by multiplex PCR. Out of 107 GNB isolates, 62 (57.9%) strains showed resistance to imipenem and/or meropenem. DDST detected MBLs in 46.8% of carbapenem resistant isolates.65.5% of phenotypically detected MβLs isolates were carrying genes for MβLs production. IMP was the most frequent detected gene (36.8%) followed by VIM, and GIM genes (31.6% for each). MβLs production were more frequent in E.coli, Pseudomonas species, Acintobacter species (26.3% for each) followed by Klebsiella species (15.8%) and Moraxella species (5.3%). Our findings showed production of MβLs in a considerable number of GNB which is alarming and need continuous surveillance to detect such resistant strains. DDST is simple method and could be routinely applied for early detection ofthis resistance to establish the proper antibiotic therapy and infection control measures to combat their spread. Future studies are needed for extensive molecular characterization of MβLsamong GNB in our locality.


Keywords: Double-disk synergy test, Gram negative bacilli, Metallo-βeta-lactamases, PCR.
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How to cite this article:

Maysaa El-Sayed Zaki, Mervat Mashaly and Reham El Agamy. 2017. Phenotypic Detection and Genotypic Characterization of Metallo-Beta-Lactamases in Gram Negative Bacilli isolated from Mansoura University Hospital in Egypt.Int.J.Curr.Microbiol.App.Sci. 6(2): 1465-1505. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.167