Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 520-526
DOI: https://doi.org/10.20546/ijcmas.2020.901.057


Isolation and Molecular Identification of Escherichia coli in Diarrheic Captive Asian Elephants (Elephas maximus) of Tamil Nadu State, India
A. Senthilkumar1*, M. G. Jayathangaraj2, A. Valli3, A. Thangavelu4, S. Gomathinayagam5 and N. Sribalaji6
1Tamil Nadu Veterinary and Animal Sciences University, Farmers Training Centre, Theni-625 531, India
2Department of Veterinary Clinical Medicine and Therapeutics, Madras Veterinary College, Chennai-600007, India
3Institute of Animal Nutrition, PGRIAS, Kattupakkam, Chennai-600007, India
4Department of Veterinary Microbiology,
5Department of Veterinary Parasitology, Madras Veterinary College, Chennai-600007, India
6MSRS, Pottaneri, India
*Corresponding author
Abstract:

The main objective of the present study was isolation and molecular identification of Escherichia coli in diarrheic captive Asian elephants (Elephas maximus) of Tamil Nadu state. Isolation and identification of E.coli was carried out in both fresh water and dung samples from diarrheic cases of captive elephants (n=8), using standard microbiological culturing method and PCR technique. Out of dung (n=8) and water (n=8) samples, 62.50% (n=5) of water samples as well as 62.50% (n=5) of the dung samples were found to be positive for Escherichia coli. On MacConkey Agar, the typical lactose fermenting foul smelling pink coloured colonies indicated the growth of E.coli. In Eosin-Methylene blue agar, the colonies appeared as blackish centres with a metallic sheen indicating the growth of E.coli. Molecular identification of E.coli by Polymerase Chain reaction (PCR), the amplicon size was 476bp.These findings in this study indicated the need for supply of good quality water to the captive elephants, in order to minimize the possible incidences of contamination of Escherichia coli.


Keywords: Asian elephants-diarrhoea- E.coli
Download this article as Download

How to cite this article:

Senthilkumar, A., M. G. Jayathangaraj, A. Valli, A. Thangavelu, S. Gomathinayagam and Sribalaji, N. 2020. Isolation and Molecular Identification of Escherichia coli in Diarrheic Captive Asian Elephants (Elephas maximus) of Tamil Nadu State, India.Int.J.Curr.Microbiol.App.Sci. 9(1): 520-526. doi: https://doi.org/10.20546/ijcmas.2020.901.057