Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 160-168
DOI: https://doi.org/10.20546/ijcmas.2020.901.018


Molecular Characterization of Potent Biocontrol Isolates of Trichoderma asperellum and Pseudomonas fluorescens from Tomato Rhizosphere
Raghuveer Singh1*, N. Tiameren Ao2, Valenta Kangjam2, Susanta Banik2, Susheel Kumar Sharma3, G. Rajesha4, Monika Hajong4, C. Lalhruaitluangi2 and L. Daiho2
1ICAR Research Complex for NEH Region, Arunachal Pradesh Centre, Basar-791101, Arunachal Pradesh, India
2Department of Plant Pathology, SASRD, Nagaland University, Medziphema-797106, Nagaland, India
3ICAR Research Complex for NEH Region, Manipur Centre, Lamphelpat-795004, Imphal, Manipur, India
4ICAR Research Complex for NEH Region, Nagaland Centre, Medziphema-797106, Nagaland, India
*Corresponding author
Abstract:

The potent native bioagents isolated from tomato rhizosphere have high antagonistic potential against Phytophthora infestans. Molecular characterization of fungal isolates (T-11 and T-14) was performed by using Internal Transcribed Spacer (ITS) region of 18S rRNA sequence. The sequence analyses of two isolates (MK928414 and MK928417), showed 100% similarity with Trichoderma asperellum during BLAST analysis. The phylogeny tree constructed by using sequence analysis showed the formation of cluster along with T. asperellum and both the isolates were identified as T. asperellum. The rhizospheric potential bacterial isolates (Pf-2 and Pf-3) were isolated and characterized at molecular level. Bacterial isolates were identified as Pseudomonas fluorescens by using 16S-23S rRNA intervening sequence specific ITS1 and ITS2 primers. The sequence analyses of two isolates showed 89.76-92.67% similarity with P. fluorescens during BLAST analysis and received the NCBI GenBank accession number of MN783298 and MN783297 for Pf-2 and Pf-3 isolates respectively. These isolates were further confirmed by phenotypic characteristics. It is inferred from the present study, that taxonomical knowledge on bioagents is important for accurate identification and molecular characterization of potential biocontrol species. This is to undeniably avoid potential risk of introducing an unknown bacterial and fungal species into the rhizosphere of a given ecosystem.


Keywords: Tomato; Trichoderma; Pseudomonas;ITS region; Phylogenetic analysis
Download this article as Download

How to cite this article:

Raghuveer Singh, N. Tiameren Ao, Valenta Kangjam, Susanta Banik, Susheel Kumar Sharma, G. Rajesha, Monika Hajong, C. Lalhruaitluangi and Daiho, L. 2020. Molecular Characterization of Potent Biocontrol Isolates of Trichoderma asperellum and Pseudomonas fluorescens from Tomato Rhizosphere. Int.J.Curr.Microbiol.App.Sci. 9(1): 160-168. doi: https://doi.org/10.20546/ijcmas.2020.901.018