Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 1325-1335
DOI: https://doi.org/10.20546/ijcmas.2019.812.161


Expression of Efflux Pump Activity in Multidrug Resistant (MDR) E. coli isolates by Cartwheel Method
Thakur Datt1*, Shyama Datt1, Iqbal Singh2, Simrita Singh3 and N. P. Singh1*
1Department of Microbiology, 2Department of Surgery, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi -110095, India
3NCDC, 22-Shamnath Marg, Delhi -110054, India
*Corresponding author
Abstract:

Bacteria resist antibiotic action through several mechanisms, including target al., teration, drug inactivation, decreased permeability, and increased efflux. The aim of the study is detection of EP mediated MDR phenotypes in E. coli by using EB agar Cartwheel method. The agar-based method is used to evaluate as many as twelve bacterial strains per plate and has been termed the EB-agar cartwheel method. E. coli strains were grown in 5 ml peptone water until they reached an optical density (OD) 0.6 at 600 nm, then OD of the cultures was adjusted to 0.5 of a McFarland standard. EB concentrations starting from 0, 0.5, 1, 1.5, 2 & 2.5 mg/l were prepared in distilled water on the same day of the experiment from stock solution (50 mg/ml, stored at 4˚C) and protected from light. The evaluation of efflux-pump activity was done on70 MDR E. coli strains isolated from urine specimens of clinically suspected UTI patients of age 18‑80 years. The prevalence of active efflux was observed in 60% (42/70) MDR E. coli strains. The high prevalence of an active efflux system in the present study in MDR E. coli isolates indicate that efflux activity-based antibiotic resistance is one of the mechanism of drug resistance prevalent in MDR E. coli isolates.


Keywords: Optical density, Antibiotic resistance, Phenotypes, Permeability, Bacteria
Download this article as Download

How to cite this article:

Thakur Datt, Shyama Datt, Iqbal Singh, Simrita Singh and Singh, N. P. 2019. Expression of Efflux Pump Activity in Multidrug Resistant (MDR) E. coli isolates by Cartwheel Method.Int.J.Curr.Microbiol.App.Sci. 8(12): 1325-1335. doi: https://doi.org/10.20546/ijcmas.2019.812.161