Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 2568-2576
DOI: https://doi.org/10.20546/ijcmas.2019.812.300


Phenotypic and Genotypic Characterization of Antimicrobial Resistance of Enterobacteriaceae Isolated from Free Range Chicken in Andhra Pradesh
M. Divya1, B. Sreedevi1*, R. K. Chaitanya1 and Ch. Srilatha2
1Department of Veterinary Microbiology,
2Department of Veterinary Pathology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, India
*Corresponding author
Abstract:

Antimicrobial resistance in bacteria from the family Enterobacteriaceae is an important indicator of the emergence of resistant bacterial strains in the gut microbiota. A total of 150 cloacal swabs were collected from free range chickens, 217 Enterobacteriaceae isolates were identified. This study investigated the antimicrobial susceptibility and resistance of commensal Enterobacteriaceae from free-range chickens to 14 antimicrobial agents using the disc diffusion method. Hundred per cent phenotypic resistance of the isolates was noticed against bacitracin, colistin, nitrofurazone, furazolidone, virginomycin, vancomycin and tylosine. Moderate resistance (70-25%) of isolates was noticed against doxycycline HCl, ampicillin, cefotaxime, ciprofloxacin and chloramphenicol. Least resistance (<25%) of isolates was recorded against enrofloxacin and gentamicin. The genotypic resistance of the Enterobacteriaceae to tetracycline was detected by targeting tet-A gene. About 90.9% of phenotypic resistant samples were found to harbour tet-A gene. Resistance to ampicillin was detected by targeting blaTEM gene and 90.55% of phenotypic resistant samples against ampicillin were found to have blaTEM gene.


Keywords: Free range chicken, Enterobacteriaceae, Antibiotic resistance, Tet A and blaTEM genes
Download this article as Download

How to cite this article:

Divya, M., B. Sreedevi, R. K. Chaitanya and Srilatha, Ch. 2019. Phenotypic and Genotypic Characterization of Antimicrobial Resistance of Enterobacteriaceae Isolated from Free Range Chicken in Andhra Pradesh.Int.J.Curr.Microbiol.App.Sci. 8(12): 2568-2576. doi: https://doi.org/10.20546/ijcmas.2019.812.300