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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 2515-2521
DOI: https://doi.org/10.20546/ijcmas.2019.812.294


Genotyping Staphylococcus aureus Isolates with RS-PCR from Chicken Meat in Chennai City
S. J. Deepak, K. Porteen*, A. Elango, T. M. A. Senthil Kumar, R. Narendra Babu, S. Sureshkannan and S. Wilfred Ruban
Department of Veterinary Public Health and Epidemiology, Madras Veterinary College, TANUVAS, Chennai 600007, India
*Corresponding author
Abstract:

The Staphylococcus aureus is a versatile opportunistic pathogen affecting both human and animals. The Genotyping of S. aureus isolates helps to understand the pathogen’s dissemination and epidemiological clusters, Hence, the present study designed to study the genotyping Staphylococcus aureus with RS-PCR from Chicken meat in Chennai City. In the present study, ribosomal spacer PCR (RS-PCR) was found to be showing polymorphism with varying band size and on software based dendrogram analysis. The dendrogram obtained upon analysis found that 3 main clusters and named as Cluster A, Cluster B and Cluster C. The similarity co-efficient at 0.05 they were characterised as 23 ribotypes. The most frequent ribotype belong to Cluster C and ribotype no 23. Based on Cluster analysis in our study revealed that may be single evolutionary originated epidemiological similar S. aureus strain is circulating and its clustered with transmitted in chicken meat though out the city thou meat was not collected from the same farm or site. The PCR ribotyping is a rapid inexpensive technique that is highly reproducible and almost as discriminatory as other typing methods for S. aureus isolates and should be useful in the local investigation and control of outbreaks.


Keywords: S. aureus, Ribosomal spacer-PCR, 16S-23S ribosomal spacer region, Phylogenetic tree, Evolutionary analysis
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How to cite this article:

Deepak, S. J., K. Porteen, A. Elango, T. M. A. Senthil Kumar, R. Narendra Babu, S. Sureshkannan and Wilfred Ruban, S. 2019. Genotyping Staphylococcus aureus Isolates with RS-PCR from Chicken Meat in Chennai City.Int.J.Curr.Microbiol.App.Sci. 8(12): 2515-2521. doi: https://doi.org/10.20546/ijcmas.2019.812.294