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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 700-707
DOI: https://doi.org/10.20546/ijcmas.2019.812.092


Molecular Characterization of Staphylococcus aureus Isolated from Foods of Animal Origin by Targeting Virulence and Antibiotic Resistance Genes
Devender Choudhary*, Surendra Singh Shekhawat, Abhishek Gaurav,
Hitesh Kumar and Nirmal Kumar
Department of Veterinary Public Health, CVAS, Navania, Vallabhnagar,
Udaipur (Raj.) -313601, India
*Corresponding author
Abstract:

Meat, milk and egg are a major component in the human diet and milk is an important food for vegetarian class, but it also serves as a very good medium for the growth of many microorganisms including pathogenic bacteria (Ruegg, 2003). In the present study, samples of milk, egg and meat were collected from different areas of Udaipur city.PCR assay was standardized for the detection of species specific genes16SrRNA gene, virulence gene (tsst gene) and antibiotic resistance gene (ermC, tetK, and aacA-aphD) of S. aureus. The prevalence of S. aureus was found to be 37.5%, 5%, and 15% in milk, egg and meat respectively. The prevalence of 16SrRNA, tsst, ermC, tetK, and aacA-aphD genes were recorded as 100%, 21.73%, 13%, 26% and 21.7% respectively.


Keywords: Milk, Meat, Egg, PCR, Virulence gene and antibiotic resistance gene
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How to cite this article:

Devender Choudhary, Surendra Singh Shekhawat, Abhishek Gaurav, Hitesh Kumar and Nirmal Kumar. 2019. Molecular Characterization of Staphylococcus aureus Isolated from Foods of Animal Origin by Targeting Virulence and Antibiotic Resistance Genes.Int.J.Curr.Microbiol.App.Sci. 8(12): 700-707. doi: https://doi.org/10.20546/ijcmas.2019.812.092