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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 2089-2098
DOI: https://doi.org/10.20546/ijcmas.2019.812.247


Occurrence and Characterization of Extended Spectrum β- lactamase Producing E. coli and Salmonella spp. from Raw Milk Samples in Wayanad District, Kerala, India
L. Akarsh2*, Prejit1, 2, K. Asha2, Jess Vergis2, V. K. Vinod2 and Hema Persis Andrews2
1Centre for One Health Education, Advocacy, Research and Training,
2Department of Veterinary Public Health, CV&AS, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad-673576, India
*Corresponding author
Abstract:

Escherichia coli and Salmonella are considerably responsible for causing gastrointestinal disorders. Emergence of Extended spectrum beta lactamase (ESBL) production among the above organisms is a great matter of concern. Hence, the present study is envisaged to know the distribution status of ESBL producing E. coli and Salmonella spp. from raw milk samples. A total of 50 raw milk samples was collected and were analyzed for the E. coli and Salmonella through conventional culture method and confirmation by PCR using uidA gene (E. coli) and invA gene (Salmonella). The occurrence of E. coli and Salmonella spp in raw milk was found to be 60 per cent and 18 per cent, respectively Further, characterization of isolates for phenotypic ESBL production revealed that 21 E. coli (70%) and 7 Salmonella (77.77 %) as phenotypic ESBL producers and were resistant to third generation cephalosporins. blaTEM, blaCTX-M and blaSHV gene was present in 56.66 per cent, 40 per cent and 26.66 per cent of the E. coli isolates and the occurrence of blaSHV, blaCTX-M and blaTEM gene in Salmonella isolates was 55. 5 percent, 44.4 per cent and 11.1 per cent, respectively. Thus the present study envisaged the need of hygienic milk production and proper antibiotic usage to prevent further spillover of resistant pathogenic organisms among public and environment.


Keywords: coli, Salmonella spp., Raw milk, ESBL, uidA, invA blaTEM, blaCTX-M, blaSHV
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How to cite this article:

Akarsh, K. L., Prejit, K. Asha, Jess Vergis, V. K. Vinod and Hema Persis Andrews. 2019. Occurrence and Characterization of Extended Spectrum β- lactamase Producing E. coli and Salmonella spp. from Raw Milk Samples in Wayanad District, Kerala, IndiaInt.J.Curr.Microbiol.App.Sci. 8(12): 2089-2098. doi: https://doi.org/10.20546/ijcmas.2019.812.247