National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Poultry industry in India is facing major economic setback due to persistent disease problems like colibacillosis. The incidence and severity extra intestinal infection like complicated chronic respiratory disease have increased rapidly. In this context the role of E.coli in causing extra intestinal infection needs to be explored. The study of virulence determinants associated with these bacteria help us in understanding the pathogenic mechanism of disease. The role of various serotypes associated with extra intestinal infection needs further studies. With this view this research was conducted on total 77 E. coli which were isolated from 228 tissue samples collected from dead birds suffering with colibacillosis. Out of 228 tissue samples a maximum numbers (41) 53.24 % of E.coli isolates were recovered from Navsari Dist. of Gujarat Followed by (36) 23.84% from Anand Dist. of Gujarat. All these 77 E. coli isolates were tested for susceptibility to eight suitable antibiotics by disc diffusion method. As per antibiogram pattern the antibiotic drug resistance was highest to Penicillin (P), 77 (100%), and Oxytetracycline (O), 77 (100%), followed by 74 (96.10%) to Ampicillin (AMP), Enrofloxacin (EX), 70 (90.90%), Streptomycin (S), 64 (83.11%), Chloramphenicol (C), 65 (84.41%), Gentamicin (GEN), 59 (76.62%) whereas only 20 (25.97%) isolates were resistant to Cefatrixone (CTR). All these isolates were tested for invasiveness by Congo red binding test, 64 (83.11%) showed red colonies after 48 hrs of incubation at room temperature and were found to be invasive, while 13 (16.88%) showed colorless colonies turned out to be negative (non-invasive) for Congo red binding test. The Serotyping of selected 43 isolates was done at NSEC, CRI, Kasauli (HP). India. The results revealed UT (6), O88 (6), O83 (4), O126 (1), O49 (3), O145 (2) and O84(5), O7(2), O157(9), O8(4), O119(1), Among these serotypes the most frequent serotypes detected were O157(9) O88 (6), UT (6), O84(5), O8(4), O49 (3), O145 (2),O7(2),.
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