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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 505-511
DOI: https://doi.org/10.20546/ijcmas.2019.812.067


Serotyping and Antimicrobial Susceptibility Pattern of Avian Pathogenic Escherichia coli
M. Muglikar1*, I. H. Kalyani1, Dhruv Desai1, J. M. Patel2, D. R. Patel1,Pushpa Makwana1 and J. B. Solanki3
1Department of Veterinary Microbiology, 2Department of Veterinary Pathology,3Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, Navsari, (Gujarat), India
*Corresponding author
Abstract:

Poultry industry in India is facing major economic setback due to persistent disease problems like colibacillosis. The incidence and severity extra intestinal infection like complicated chronic respiratory disease have increased rapidly. In this context the role of E.coli in causing extra intestinal infection needs to be explored. The study of virulence determinants associated with these bacteria help us in understanding the pathogenic mechanism of disease. The role of various serotypes associated with extra intestinal infection needs further studies. With this view this research was conducted on total 77 E. coli which were isolated from 228 tissue samples collected from dead birds suffering with colibacillosis. Out of 228 tissue samples a maximum numbers (41) 53.24 % of E.coli isolates were recovered from Navsari Dist. of Gujarat Followed by (36) 23.84% from Anand Dist. of Gujarat. All these 77 E. coli isolates were tested for susceptibility to eight suitable antibiotics by disc diffusion method. As per antibiogram pattern the antibiotic drug resistance was highest to Penicillin (P), 77 (100%), and Oxytetracycline (O), 77 (100%), followed by 74 (96.10%) to Ampicillin (AMP), Enrofloxacin (EX), 70 (90.90%), Streptomycin (S), 64 (83.11%), Chloramphenicol (C), 65 (84.41%), Gentamicin (GEN), 59 (76.62%) whereas only 20 (25.97%) isolates were resistant to Cefatrixone (CTR). All these isolates were tested for invasiveness by Congo red binding test, 64 (83.11%) showed red colonies after 48 hrs of incubation at room temperature and were found to be invasive, while 13 (16.88%) showed colorless colonies turned out to be negative (non-invasive) for Congo red binding test. The Serotyping of selected 43 isolates was done at NSEC, CRI, Kasauli (HP). India. The results revealed UT (6), O88 (6), O83 (4), O126 (1), O49 (3), O145 (2) and O84(5), O7(2), O157(9), O8(4), O119(1), Among these serotypes the most frequent serotypes detected were O157(9) O88 (6), UT (6), O84(5), O8(4), O49 (3), O145 (2),O7(2),.


Keywords: Ampicillin (AMP), Enrofloxacin (EX), Aerobactin, Iroproteins, Yersiniabactin
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How to cite this article:

Muglikar, D. M., I. H. Kalyani, Dhruv Desai, J. M. Patel, D. R. Patel, Pushpa Makwana and Solanki, J. B. 2019. Serotyping and Antimicrobial Susceptibility Pattern of Avian Pathogenic Escherichia coli.Int.J.Curr.Microbiol.App.Sci. 8(12): 505-511. doi: https://doi.org/10.20546/ijcmas.2019.812.067