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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 2487-2502
DOI: https://doi.org/10.20546/ijcmas.2019.811.287


Studies on Lactate Dehydrogenase (LDH) Activity in the Pupal and Adult Stages of the Silkworm Bombyx Mori L.
M.N. Ramya* and T.S. Jagadeesh Kumar
Silkworm Physiology and Biochemistry Laboratory,Department of Studies in Sericulture Science, Manasagangothri, University of Mysore, Mysuru, Karnataka, India
*Corresponding author
Abstract:

The present study was carried out to understand the Lactate dehydrogenage (LDH) activity in the pupal and adult stages in four popular silkworm bivoltine breeds namely, CSR2, NB4D2, APS45 and APS12 and four multivoltine races namely, PM, C.nichi, APM1 and APM3 were collected from the Germplasm Bank of Department of Studies in Sericultural Sciences, University of Mysore, Mysuru, and APSSRDI, Hindupur, Andhra Pradesh. Layings were prepared and the eggs were incubated at 25±10c temperature and reared by adopting the standard methods. The male and female silkworm were separated during 5th instar just after resuming from 4th moult before feeding to get virgin female and unmated male and to identify the male and female pupae on 3rd day after spinning during pupal stage. Further, the good cocoons were selected for further assessment and utilization. The fat body samples were extracted from male and female pupal stage of 3rd day to till last day of pupal duration. Virgin and mated male and female of the silkworm adult stage from 1st day to till last day of adult life span. The extracted samples were kept at -20oc for preservation and further, utilization for the analysis of enzyme profiles of Lactate dedyhrogenase (LDH).The results obtained from the present study clearly showed, the activity profiles of the fat body LDH is a dynamic enzyme depicted the maximum level of activity in each and every day of male and female pupation period observed a sequential quantum of differences in the order of marginal increase were noticed. Further, the LDH enzyme activity profile was observed more in virgin female moth of bivoltine races/breeds than multivoltine races. However, the enzymes are very active for the synthesis of reactive oxygen species leads to the release of free radicals and even antioxidant accelerates the rate of reaction under the extreme environmental conditions. The results obtained from the present investigation are the document for an empirical analysis in the fat body tissue of healthy pupae and adult stages of different breeds/races of the silkworm Bombyx mori L.


Keywords: LDH, Activity profile, Bivoltine, multivoltine, Silkworm, Bombyx mori L.
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How to cite this article:

Ramya, M.N. and Jagadeesh Kumar, T.S. 2019. Studies on Lactate Dehydrogenase (LDH) Activity in the Pupal and Adult Stages of the Silkworm Bombyx Mori L.Int.J.Curr.Microbiol.App.Sci. 8(11): 2487-2502. doi: https://doi.org/10.20546/ijcmas.2019.811.287