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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 2350-2362
DOI: https://doi.org/10.20546/ijcmas.2019.811.272


Molecular Detection of Some Virulence Genes of Staphylococcus aureus Isolates Associated with Bovine Mastitis in Arid and Semi-Arid Regions of India
Taruna Bhati1*, Kumar Gaurav2, Sunita Choudhary2, Diwakar and Anil Kumar Kataria1
1Department of Veterinary Microbiology and Biotechnology, College of Veterinary and Animal Science, RAJUVAS, Bikaner-334 001, Rajasthan, India
2Department of Veterinary Microbiology, Aravali Veterinary College, Sikar, India
3Center for Studies on Wildlife Management and Health, RAJUVAS, Bikaner, India
*Corresponding author
Abstract:

Staphylococcus aureus (S. aureus) is one of the most common causes of contagious bovine mastitis and its different virulence factors involved in the pathogenesis are well documented worldwide. The present study was conducted in 107 isolates of S. aureus recovered from mastitic milk (n=51), udder surfaces (n=35) and milkers’ hands (n=21) for detection and characterization of genes encoding for hemolysins (hla, hlb and hld), leucocidin (luk-PV), surface proteins (clfA, clfB, fnbA, fnbB) and penicillin resistance (blaZ) by multiplex PCR. All the isolates were positive for blaZ, hla, hld and clfA genes while 94.4%, 85%, 63.6%, 44.9% and 19.6% isolates carried clfB, hlb, fnbA, fnbB and luk-PV genes, respectively. Mastitic milk isolates showed higher frequency of clfB (96.07%) and hlb (88.2%) genes, udder isolates for fnbB (48.6%) and luk-PV (42.9%) genes while isolates of human origin showed higher occurrence of fnbA (66.7%) genes. An overall high prevalence of hemolysins, surface proteins, adhesins, and penicillin resistance genes was observed. A little association was seen between presence of hemolysin and blaZ genes and their phenotypic expression. The recovery of penicillin resistant S. aureus strains from bovine mastitis and close human contacts in India is of concern in clinical management of mastitis.


Keywords: Bovine mastitis, Staphylococcus aureus, Hemolysins, Adhesins, Penicillin resistance
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How to cite this article:

Taruna Bhati, Kumar Gaurav, Sunita Choudhary, Diwakar and Anil Kumar Kataria. 2019. Molecular Detection of Some Virulence Genes of Staphylococcus aureus Isolates Associated with Bovine Mastitis in Arid and Semi-Arid Regions of India.Int.J.Curr.Microbiol.App.Sci. 8(11): 2350-2362. doi: https://doi.org/10.20546/ijcmas.2019.811.272