National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Staphylococcus aureus (S. aureus) is one of the most common causes of contagious bovine mastitis and its different virulence factors involved in the pathogenesis are well documented worldwide. The present study was conducted in 107 isolates of S. aureus recovered from mastitic milk (n=51), udder surfaces (n=35) and milkers’ hands (n=21) for detection and characterization of genes encoding for hemolysins (hla, hlb and hld), leucocidin (luk-PV), surface proteins (clfA, clfB, fnbA, fnbB) and penicillin resistance (blaZ) by multiplex PCR. All the isolates were positive for blaZ, hla, hld and clfA genes while 94.4%, 85%, 63.6%, 44.9% and 19.6% isolates carried clfB, hlb, fnbA, fnbB and luk-PV genes, respectively. Mastitic milk isolates showed higher frequency of clfB (96.07%) and hlb (88.2%) genes, udder isolates for fnbB (48.6%) and luk-PV (42.9%) genes while isolates of human origin showed higher occurrence of fnbA (66.7%) genes. An overall high prevalence of hemolysins, surface proteins, adhesins, and penicillin resistance genes was observed. A little association was seen between presence of hemolysin and blaZ genes and their phenotypic expression. The recovery of penicillin resistant S. aureus strains from bovine mastitis and close human contacts in India is of concern in clinical management of mastitis.
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