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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 779-789
DOI: https://doi.org/10.20546/ijcmas.2019.811.092


Evaluation of a Multiplex PCR Assay for Direct Detection of Microbial DNA from Whole Blood of Suspected Cases of Late Onset Neonatal Sepsis in a Tertiary Care Hospital of Western Maharashtra, India
Mohit Wadhi1*, Abhay Chowdhary1, Chhaya Chande1 and Sonal Thavare2
1Department of Microbiology, Grant Government Medical College & Sir JJ Group of Hospitals, Byculla, Mumbai-400008, Maharashtra, India
2Department of Microbiology, Lokmanya Tilak Municipal Medical College and General Hospital, Sion, Mumbai, Maharashtra, India
*Corresponding author
Abstract:

Neonatal sepsis is one of the leading causes of neonatal mortality in India. There has been a reduction in early-onset sepsis due to advances in obstetric care while incidence of late-onset has increased especially in very low birth weight infants. Diagnosis of neonatal sepsis remains difficult due to non-specific signs and symptoms in neonates and delay in diagnosis leads to complications. Although blood culture has been the gold standard in detection of bloodstream pathogens it lacks rapidity and sensitivity which are overcome by molecular techniques. In the present prospective study conducted from April 2017 to March 2018, a multiplex PCR assay was evaluated for detection of pathogen DNA directly from whole blood of neonates with late-onset sepsis in a tertiary care hospital. Blood culture was positive in 71/210 (33.81%) neonates with Coagulase negative Staphylococcus species being the most common isolate (28.38%). PCR added a statistically significant advantage in sensitivity (40.71% vs 37.14%) and negative predictive value over blood culture and also added a diagnostic yield by detecting additional 17 isolates that were missed by conventional blood culture. PCR and blood culture can identify a partially overlapping set of bloodstream infections making PCR a useful adjunct to blood culture.


Keywords: Late-onset neonatal sepsis, Blood culture, Multiplex PCR
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How to cite this article:

Mohit Wadhi, Abhay Chowdhary, Chhaya Chande and Sonal Thavare. 2019. Evaluation of a Multiplex PCR Assay for Direct Detection of Microbial DNA from Whole Blood of Suspected Cases of Late Onset Neonatal Sepsis in a Tertiary Care Hospital of Western Maharashtra.Int.J.Curr.Microbiol.App.Sci. 8(11): 779-789. doi: https://doi.org/10.20546/ijcmas.2019.811.092