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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 474-484
DOI: https://doi.org/10.20546/ijcmas.2019.811.058


Isolation and Characterization of the Major Outer Membrane Protein (OMP) of Pasteurella multocida Serotype B:2
Joycee Jogi*, Anju Nayak, P.C. Shukla, Amita Dubey, Ranvijay Singh, Ajay Rai and Poonam Shakya
Department of Veterinary Microbiology, College of Veterinary Science & A.H., NDVSU, Jabalpur, India
*Corresponding author
Abstract:

Outer membrane proteins (OMPs) play a significant role in the pathogenesis of Pasteurellosis. Outer membrane proteins of P. multocida are reported to be immunogenic and protective. OMPs of Pasteurella multocida (P. multocida) B:2 vaccine strain P52 was characterized by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) technique. The P. multocida P52 OMP demonstrated the presence of six OMP bands. The major bands were of 20, 34, 48, 72, 89 and 91 kDa. Potential immunogens of P. multocida P52 were identified by electroblot immunoassay. Immunoblotting was performed using the hyperimmune serum raised against the whole cell antigen. Polypeptides separated on 12% SDS-PAGE were transferred to nitrocellulose membrane by semi-dry system. Presence of three polypeptides of 34, 72 and 91 kD size was reported showing reaction with antiserum. A single band of 34kDa in SDS-PAGE was evident and was confirmed by western blot analysis.


Keywords: Pasteurella multocida, SDS-PAGE, Outer membrane protein
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How to cite this article:

Joycee Jogi, Anju Nayak, P.C. Shukla, Amita Dubey, Ranvijay Singh, Ajay Rai and Poonam Shakya 2019. Isolation and Characterization of the Major Outer Membrane Protein (OMP) of Pasteurella multocida Serotype B:2.Int.J.Curr.Microbiol.App.Sci. 8(11): 474-484. doi: https://doi.org/10.20546/ijcmas.2019.811.058