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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 1854-1868
DOI: https://doi.org/10.20546/ijcmas.2019.811.218


Molecular Detection of the Causative Agent of Soft Rot (Pectobacterium carotovorum subsp carotovorum) in Banana (Musa sp.)
G. Ragavi1, M. Muthamilan1*, S. Nakkeeran1, N. Kumaravadivel2, U. Sivakumar3 and A. Suganthi4
1Department of Plant Pathology, CPPS,
2Department of Plant Molecular Biology and Bioinformatics, Centre for Plant Molecular Biology and Biotechnology,
3Department of Agricultural Microbiology,
4Department of Agricultural Entomology, TNAU, Coimbatore, India
*Corresponding author
Abstract:

Soft rot disease of Banana caused by Pectobacterium carotovorum subsp. Carotovorum is found to be a serious disease and cause considerable yield loss. Common cultivars viz., Grand Naine, Rasthali and Nendran are extremely prone to this disease. Understanding the disease diagnosis and pathogen virulence is important in management programs. The pathogen was isolated from infected rhizomes from five locations of Theni district of Tamil Nadu. Pathogenicity of all isolates was confirmed by in vitro inoculation in rhizome bits and in vivo inoculation in pseudostems of three months old tissue cultured plants of cv. Grand Naine. The virulent isolates Icb4 and Ikg2 were characterized according to the morphological and microscopic characteristics, along with the physiological and biochemical assays; which indicates that the bacteria are gram negative, rod shaped, peritrichously flagellated. The colonies form characteristic deep cavities or pits in Crystal violet pectate medium (CVP). Results of biochemical tests showed that isolates Icb4 and Ikg2 have a positive reaction for catalase, H2S production and nitrate reductase and a negative reaction for oxidase test, Indole test and gelatin liquefaction. The results determining the optimal temperature for the growth of isolates Icb4, and Ikg2 have revealed that the temperature at 30 ºC - 37 ºC were suitable to obtain profuse growth of Pectobacterium. Total DNA extracted from the isolates Icb4, and Ikg2 were subjected to PCR analysis of 16S-23S intergenic transcribed spacer region. PCR amplified 1.5 kb amplicons were sequenced. Sequence Homology and phylogeny analyses had shown that the isolates Icb4 and Ikg2 were belonged to Pectobacterium carotovorum subsp. carotovorum.


Keywords: Banana (Musa sp), Pectobacterium carotovorum subsp. carotovorum, Soft rot
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How to cite this article:

Ragavi, G., M. Muthamilan, S. Nakkeeran, N. Kumaravadivel, U. Sivakumar and Suganthi, A. 2019. Molecular Detection of the Causative Agent of Soft Rot (Pectobacterium carotovorum subsp carotovorum) in Banana (Musa sp.).Int.J.Curr.Microbiol.App.Sci. 8(11): 1854-1868. doi: https://doi.org/10.20546/ijcmas.2019.811.218