National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Soft rot disease of Banana caused by Pectobacterium carotovorum subsp. Carotovorum is found to be a serious disease and cause considerable yield loss. Common cultivars viz., Grand Naine, Rasthali and Nendran are extremely prone to this disease. Understanding the disease diagnosis and pathogen virulence is important in management programs. The pathogen was isolated from infected rhizomes from five locations of Theni district of Tamil Nadu. Pathogenicity of all isolates was confirmed by in vitro inoculation in rhizome bits and in vivo inoculation in pseudostems of three months old tissue cultured plants of cv. Grand Naine. The virulent isolates Icb4 and Ikg2 were characterized according to the morphological and microscopic characteristics, along with the physiological and biochemical assays; which indicates that the bacteria are gram negative, rod shaped, peritrichously flagellated. The colonies form characteristic deep cavities or pits in Crystal violet pectate medium (CVP). Results of biochemical tests showed that isolates Icb4 and Ikg2 have a positive reaction for catalase, H2S production and nitrate reductase and a negative reaction for oxidase test, Indole test and gelatin liquefaction. The results determining the optimal temperature for the growth of isolates Icb4, and Ikg2 have revealed that the temperature at 30 ºC - 37 ºC were suitable to obtain profuse growth of Pectobacterium. Total DNA extracted from the isolates Icb4, and Ikg2 were subjected to PCR analysis of 16S-23S intergenic transcribed spacer region. PCR amplified 1.5 kb amplicons were sequenced. Sequence Homology and phylogeny analyses had shown that the isolates Icb4 and Ikg2 were belonged to Pectobacterium carotovorum subsp. carotovorum.
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