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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 908-919
DOI: https://doi.org/10.20546/ijcmas.2019.811.106


In Vitro Evaluation of Fungicides against Aspergillus niger causing Collar Rot Disease in Groundnut (Arachis hypogaea L.)
Amritpal Singh Sekhon*, Prabhjodh Singh Sandhu, Pankaj Sharma and Rakesh Belludi
Department of Plant Pathology, Punjab Agricultural University, Ludhiana, 141004, Punjab, India
*Corresponding author
Abstract:

The present study was carried out to evaluate the efficacy of six fungicides against Aspergillus niger using two in vitro methods namely poisoned food technique and paper towel method. Poisoned food technique was carried out by making potato dextrose agar medium poisoned with different concentrations of fungicides followed by inoculation with actively growing culture of A. niger. Among the fungicides tested by using above method, tebuconazole at concentrations of 25, 50, 100 and 200 ppm and azoxystrobin at concentrations of 100 and 200 ppm, completely (100%) inhibited the mycelial growth of A. niger followed by carbendazim 25% + mancozeb 50% (Sprint) @ 200 ppm (94.38%). In paper towel method, two varieties of groundnut namely SG-99 and M-522 were tested. In this method, seeds from previously collar rot infected plants were taken and treated with fungicides, then wrapped between two paper towels and these were kept in incubator. The minimum seed per cent mortality and seed rots due to A. niger was observed in seed treatment with tebuconazole followed by azoxystrobin and carbendazim 25% + mancozeb 50% (Sprint) in both of the tested varieties. In both methods, three fungicides namely tebuconazole, azoxystrobin and carbendazim 25% + mancozeb 50% (Sprint) found most effective against A. niger.


Keywords: Aspergillus niger, Fungicides, Groundnut, Collar rot, Potato dextrose agar medium
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How to cite this article:

Amritpal Singh Sekhon, Prabhjodh Singh Sandhu, Pankaj Sharma and Rakesh Belludi. 2019. In Vitro Evaluation of Fungicides against Aspergillus niger causing Collar Rot Disease in Groundnut (Arachis hypogaea L.).Int.J.Curr.Microbiol.App.Sci. 8(11): 908-919. doi: https://doi.org/10.20546/ijcmas.2019.811.106