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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 341-361
DOI: https://doi.org/10.20546/ijcmas.2019.811.043


Expression of TLR-22 Gene and Response of Non Specific Immunity of Labeo rohita Fed with Fucoidan Rich Seaweed Extract in Conjugation with Bacillus subtilis
R. Ghoshal* and N.P. Sahu
Department of Fish Nutrition, Physiology and Biochemistry, ICAR- Central Institute Of Fisheries Education, Mumbai, India
*Corresponding author
Abstract:

A 60-days feeding trial was conducted to study the combined effect of fucoidan rich seaweed extract (FRSE) from Sargassum wightii and probiotic (Bacillus subtilis) on expression of TLR22 gene in Labeo rohita fingerlings. Two hundred and ten fingerlings of uniform size group were distributed into seven experimental groups in triplicates. Each tank was stocked with ten fish and fed to satiation with iso-nitrogenous (35.33-35.6 CP%) and iso-caloric (393.33 to 399.43 Kcal/100g) purified diets containing 0% FRSE and no probiotic (control), 2% FRSE (T1), 108 CFU g-1 probiotic (T2), 1% FRSE + 105 CFU g-1 probiotic (T3), 2% FRSE + 105 CFU g-1 probiotic (T4), 1% FRSE + 108 CFU g-1 probiotic (T5) and 2% FRSE + 108 CFU g-1 probiotic (T6). After feeding trial, the experimental fish were challenged with Aeromonas hydrophila. Expression of TLR22 gene was studied in the liver and recorded significantly higher in T6 group in pre-challenge condition whereas both T6 and T4 groups during post-challenge condition. Total leucocyte count were estimated and recorded significantly higher (p<0.05) in treatment groups compared to the control with highest values in T6 group whereas albumin/globulin (A/G) ratio exhibited decreasing trend in the treatment groups. These results suggested that addition of probiotic at 105 or 108 CFU g-1 dosage with 2% FRSE act synergistically and enhanced the non specific immunity of L. rohita fish effectively.


Keywords: Fucoidan, Probiotic, Non specific immunity, A. hydrophila
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How to cite this article:

Ghoshal, R. and Sahu, N.P. 2019. Expression of TLR-22 Gene and Response of Non Specific Immunity of Labeo rohita Fed with Fucoidan Rich Seaweed Extract in Conjugation with Bacillus subtilis.Int.J.Curr.Microbiol.App.Sci. 8(11): 341-361. doi: https://doi.org/10.20546/ijcmas.2019.811.043