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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 988-996
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.111


Bioconversion of Amino Acids to α-Keto Acid by Geotrichum candidum Used for the Treatment of Chronic Kidney Disorders
Ketaki Chauhan1*, B.R.M. Vyas1, Anamik K. Shah2 and Von Johnson3
1Department of Biosciences, Saurashtra University, Rajkot, Gujarat, India- 360005
2Gujarat Vidhyapith, Ahmedabad, Gujarat, India- 360005
3Enformtech, 19 Laureston Avenue, Papatoetoe, Auckland, New Zealand
*Corresponding author
Abstract:

The present study explores the bioconversion by Geotrichum candidum of five amino acids to their corresponding α-keto acids used in the treatment of chronic kidney disorder. The amino acid oxidase activity biotransforming amino acids to α-keto acids was detected in biomass as well as extracellular culture fluids of G. candidum. Bioconversion was determined by measuring α-keto acid production along with residual amino acid. There was a 2-fold increase in the amount of α-keto acids produced over a period of 24 hours by permeabilization of biomass when compared to immobilized biomass. Permeabilized G. Candidum biomass was further immobilized by entrapment in Na-alginate and studied for the production of α-keto acids from the corresponding amino acids. The permeabilized and immobilized biomass beads showed an additional 2-fold increase in the α–keto acids production as compared to only permeabilized cells. Decomposition of hydrogen peroxide formed in the enzymatic reaction by manganese oxide incorporated in sodium alginate beads further improved the yield. The bioconversion efficiency of the studied amino acids to their corresponding keto acids obtained in the presence of MnO2 by immobilized biomass of G. candidum at 4 h was found to be 29-34%.


Keywords: Geotrichum candidum, Amino acids, Keto acids, Kidney disorder, Permeabilization, Immobilization
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How to cite this article:

Ketaki Chauhan, B.R.M. Vyas, Anamik K. Shah, Von Johnson. 2017. Bioconversion of amino acids to α-keto acid by Geotrichum candidum used for the treatment of chronic kidney disorders.Int.J.Curr.Microbiol.App.Sci. 6(2): 988-996. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.111