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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 579-589
DOI: http://dx.doi.org/10.20546/ijcmas.2016.502.065


Activity of Glucose Isomerase from Bacillus thuringiensis under Different Treatments
Hamed M. El-Shora*, Ahmed M El-Shobaky and Jehan E Ghoneim
Botany Department, Faculty of Science, Mansoura University, Egypt
*Corresponding author
Abstract:

Bacillus thuringiensis is a Gram-positive bacterium naturally found in soil, water and grain dust, and can be cultivated in liquid, solid and semi-solid media. Glucose isomerase (EC. 5.3.1.5) catalyzes the reversible isomerization of glucose to fructose and that of xylose to xylulose. It is an important enzyme used in the industrial production of high-fructose corn syrup (HFCS). Glucose isomerase was purified from Bacillus thuringiensis. The final purification resulted in a considerably high yield (64.6%) with about 15.8-fold. The optimum temperature and pH were 50 C and 7.0. The optimum glucose concentration was 8 mM. Glucose isomerase required Mn2+ or Ca2+ as cofactor. The enzyme was activated by cysteine, ascorbic acid, folic acid and sodium sulfate. The enzyme was inhibited by sodium bromide, sodium azide, sodium fluoride, sodium arsenate, glycine, tyrosine, phenylalanine, arginine, asparagine, and guanidine hydrochloride. Pyruvate, glyoxylate and 2-oxoglutarate inhibited the enzyme activity at the higher concentrations.


Keywords: Glucose isomerase, Purification, Bacillus thuringiensis, characteristics.
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How to cite this article:

Hamed M El-Shora, Ahmed M El-Shobaky and Jehan E Ghoneim. 2016. Activity of Glucose Isomerase from Bacillus thuringiensis under Different TreatmentsInt.J.Curr.Microbiol.App.Sci. 5(2): 579-589. doi: http://dx.doi.org/10.20546/ijcmas.2016.502.065