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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 1917-1925
DOI: https://doi.org/10.20546/ijcmas.2019.810.223


N-terminal Domain of tlyA from mycobacterium tuberculosis Displayed Concentration Dependent Ordered Structure
V.B. Shivaleela1, Srihari Prathapaneni2, P. Sharada1* and K. Giri Gowda2
1Department of Biotechnology, Basaveshwara Engineering College,Bagalkot-587102, Karnataka, India
2Sampoorna International Institute of Agri. Science & Horticulture Technology, Mandya-571433, Karnataka, India
*Corresponding author
Abstract:

Mycobacterium tuberculosis (Mtb), the causative agent of the disease tuberculosis, is an ancient pathogen and a major cause of death worldwide. Although various virulence factors of M. tb have been identified, its pathogenesis remains incompletely understood. TlyA is a virulence factor that is evolutionarily conserved in many gram-positive bacteria, but its full length structure and function in the pathogenesis of infection with Mtb has not been elucidated. In the present study, we cloned, expressed and purified N-terminal domain of tlyA, which play a crucial role in the binding of the co-substrate S-adenosyl-L-methionine. We characterized the protein by SDS-PAGE and Circular Dichroism. TlyA model generated using tlyA crystal structure, clearly indicates E59 separates between N-terminal domain (NTD) and C-terminal domain (CTD). 


Keywords:

Mycobacterium tuberculosis, N-terminal domain, Circular dichroism, tlyA, Maltose binding protein

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How to cite this article:

Shivaleela, V.B., Srihari Prathapaneni, P. Sharada and Giri Gowda, K. 2019. N-terminal Domain of tlyA from Mycobacterium tuberculosis Displayed Concentration Dependent Ordered Structure.Int.J.Curr.Microbiol.App.Sci. 8(10): 1917-1925. doi: https://doi.org/10.20546/ijcmas.2019.810.223