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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 1801-1811
DOI: https://doi.org/10.20546/ijcmas.2019.810.209


Multiple Gene Characterization of Rice Orange Leaf (ROL)Phytoplasma Infecting Rice
Valarmathi1* and D. Ladhalakshmi2
1Department of Plant Pathology, ICAR- Central Institute for Cotton Research (CICR), Regional Station, Coimbatore-641 003, India
2 Department of Plant Pathology, ICAR-Indian Institute of Rice Research (IIRR), Hyderabad-500 030, India
*Corresponding author
Abstract:

Phytoplasmas are intracellular obligate prokaryotes that lack cell walls and have very small genomes (680–1,600 kb). Many fungal, bacterial and viral diseases have been characterized and resistant breeding programme is progressing very well. Recently, on the basis of the symptoms and on 16SrDNA sequence identity and phylogenetic relationships the association of ‘Ca. P. asteris’ with Rice Orange Leaf phytoplasma disease in South India was confirmed. Rice orange leaf phytoplasma was characterized based on multiple gene systems. The 16S-23S rRNA Spacer region of rice phytoplasma was amplified by the primer pairs p1/p7 and p3/p7 at ~320 bp from all rice samples. The phytoplasma infecting rice samples were confirmed as group I by the group specific primer for 16SrI which amplified DNA fragment at ~1.1 kb in all the samples. The tuf gene in phytoplasma (~1000 bp) and phytoplasma in rice belonging to aster yellow (AY) group was confirmed by the primer specific for aster yellow tuf gene with amplification at ~940 bp. The primer pair, AYsecYF1 and AYsecYR1 was used which amplified the secY gene at ~1.4 kb in all the rice samples which confirmed the phytoplasma infecting rice belonged to aster yellow group. This is the first report to study multiple gene systems of Rice Orange Leaf (ROL) phytoplasma infecting Rice crop.


Keywords: Rice Orange Leaf (ROL) phytoplasma, 16S-23S rRNA Spacer, 16SrI- tuf gene, secY gene
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How to cite this article:

Valarmathi, P.  and Ladhalakshmi, D. 2019. Multiple Gene Characterization of Rice Orange Leaf (ROL) Phytoplasma Infecting Rice.Int.J.Curr.Microbiol.App.Sci. 8(10): 1801-1811. doi: https://doi.org/10.20546/ijcmas.2019.810.209