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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 840-851
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.094


Biochemical Identification of Protease Producing Bacterial Isolates from Food Industries by Vitek 2 Compact System
I.S. Sony* and V.P. Potty
Department of Microbiology, Cashew Export promotion Council of India (CEPCI),Mundakkal, Kollam, Kerala, India
*Corresponding author
Abstract:

The objective of the present study was to identify protease producing bacteria isolated from food processing industries. Isolation of the organism was performed by serial dilution agar plate technique and initial screening of protease production was done using gelatine agar plates by flooded the plates with mercuric chloride solution (HgCl2-15g and 20 ml of 6.0 N HCl made up to 100 ml with distilled water). A total of 5 isolates were selected based on zone diameter in gelatine clear zone method and named as TKMFT8, TKMFT22, TKMFT25, TKMFT39 and TKMFT53. Initial identification of the selected isolates was carried out using cultural characterization, microscopic observation and Biomerieux VITEK 2 system identification based on biochemical reactions and identified as Staphylococcus sciuri (TKMFT8, TKMFT22, TKMFT25, and TKMFT39) and Achromobacter xylosoxidans (TKMFT53). Protease producing bacterial isolates can be used for the degradation proteinaceous waste material from food manufacturing units leading to recycling of food industry waste.


Keywords: Protease, Biomerieux VITEK 2 system, Staphylococcus sciuri, Achromobacter xylosoxidans.
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How to cite this article:

Sony, I.S., and Potty, V.P. 2017. Biochemical Identification of Protease Producing Bacterial Isolates from Food Industries by Vitek 2 Compact SystemInt.J.Curr.Microbiol.App.Sci. 6(2): 840-851. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.094