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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 947-959
DOI: https://doi.org/10.20546/ijcmas.2019.810.111


Influence of Nitrogen Source on the Catabolism of Naphthalene by a Pseudomonas aeruginosa TU
K.R. Savitha1, T.N. Shamu1, Lalitha Junna2 and Manohar Shinde1*
1Department of Studies and Research in Biochemistry, Tumkur University, Tumakuru-572103, Karnataka, India
2Department of Biochemistry, Gulbarga University, Kalaburagi-585106, Karnataka, India
*Corresponding author
Abstract:

A Pseudomonas aeruginosa TU isolated from diesel-contaminated site degraded 66% of an initial 10 mM naphthalene load after 48 h of incubation in a minimal-salts medium containing NH4Cl as nitrogen source whereas, the same bacterium, when incubated in the minimal-salts medium containing KNO3,degraded 10 and 20 mM initial naphthalene load within in 24 and 36 h respectively. The naphthalene enrichment medium containing NH4+ as nitrogen source slowed bacterial growth and compromised cell viability over 72 h while, naphthalene-minimal salts medium when supplemented by NO3-showed uniform and efficient cell growth. The bacterial degradation of naphthalene in the minimal-salts medium containing KNO3 resulted in transient accumulation of salicylate (0.11mM) and catechol (102 µM) after 12 and 24 h respectively whereas, in the medium containing NH4Cl, the naphthalene biodegradation caused decrease in the pH of culture medium from 7 to 3.8 and accumulation of 0.63 mM salicylate and 24 µM catechol as dead end products. Further, the cell-free extracts of the bacterium grown on naphthalene in the medium containing KNO3showed high activities of naphthalene-degrading enzyme as compared to that of the cell-free extracts of bacterium grown on naphthalene and NH4Cl.


Keywords: Pseudomonas aeruginosa TU, Nitrogen source, Naphthalene-degrading enzyme
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How to cite this article:

Savitha, K.R., T.N. Shamu, Lalitha Junna and Manohar Shinde. 2019. Influence of Nitrogen Source on the Catabolism of Naphthalene by a Pseudomonas aeruginosa TU.Int.J.Curr.Microbiol.App.Sci. 8(10): 947-959. doi: https://doi.org/10.20546/ijcmas.2019.810.111