International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:2, February, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(2): 557-566

Genetic Purity of Flue Cured Tobacco F1 Hybrid with RAPD Markers
Satish Kumar* and D. Kalyani
School of Life and Health Sciences, Adikavi Nannaya University, Rajah Rajah Narendhra Nagar, Rajamahendravaram, Andhra Pradesh 533296, India
*Corresponding author

Tobacco (Nicotiana tabacum) crop is grown for its leaves and it is covered in an area of 4 million hectares in 125 countries. India is the second largest producer of tobacco and is grown in different states. In India Flue Cured Virginia (FCV) and non-flue cured tobacco varieties were cultivated. Genetic purity in hybrid tobacco is the major constraint. Twenty RAPD primers were used to identify the tobacco parents and its F1 hybrid out of twenty RAPD’s, three primers (OPA-5, OPA-7 and OPB-9) were amplified specific to male parent, female parent and its F1 hybrid OPA-7 primer showed polymorphism for male parent, female parent and its hybrid. Fifty seed lots (1-50) were tested with OPA-7 primer and found a few off types in the three seed lots. The genetic purity in seed lot-6 (S) is 97%, seed lot-14 (T) is 96% and seed lot-31 (U) is 98%. The cost effective field grow out trials (GOT) were replaced by this RAPD analysis and the results were obtained in less time

Keywords: Genetic purity, Tobacco hybrids, RAPD, GOT, Seed lots, off types

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How to cite this article:

Satish Kumar, D.,  and Kalyani, D. 2016. Genetic Purity of Flue Cured Tobacco F1 Hybrid with RAPD Markers.Int.J.Curr.Microbiol.App.Sci. 5(2): 557-566. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.