International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:6, June, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(6): 3912-3922

Characterization of Sclerotium rolfsii Sacc. causing Collar Rot in Chickpea Isolates using Cultural and Morphological Traits
P. V. Srividya1, M. Lal Ahamed1, J. V. Ramana1* and S. Khayum Ahmmed2
1Department of Molecular Biology and Biotechnology, APGC, Lam, Guntur, India 2Department of Plant Pathology, AICRP on Chickpea, RARS, Nandyal, India
*Corresponding author

Collar rot is one of the major diseases of chickpea caused by Sclerotium rolfsii. In the present study, morphological and cultural variability of 20 isolates of Sclerotium rolfsii isolated from major chickpea growing areas of Andhra Pradesh were studied based on their growth rate, colony colour and appearance and Sclerotium colour, arrangement and maturity days of sclerotia using two solid media viz., Potato Dextrose Agar and Czapex Dok Agar. The isolates, CSR 14, CSR 18 and CSR 20, had very fast growth on both PDA and CDA. The overall growth of the isolates was more (dense mycelial formation) on PDA compared to CDA. The site of sclerotia formation and its growth were varied with the isolate and most of the isolates showed peripheral formation in CDA while it was scattered in PDA. The isolate, CSR 14, showed light orange coloured sclerotial bodies formation in the central region on both PDA and CDA. Thus, the present demonstrated the existence of variability among the isolates and development of effective management strategies to overcome the disease at early crop growth period.

Keywords: Cultural, Morphological characterization, PDA, CDA, S. rolfsii

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How to cite this article:

Srividya, P. V., M. Lal Ahamed, J. V. Ramana and Khayum Ahmmed, S. 2018. Characterization of Sclerotium rolfsii Sacc. causing Collar Rot in Chickpea Isolates using Cultural and Morphological Traits.Int.J.Curr.Microbiol.App.Sci. 7(6): 3912-3922. doi:
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