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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 2585-2589
DOI: https://doi.org/10.20546/ijcmas.2019.809.299


Expression of pcDNA-Interferon Lambda-3 (pcDNA-IFNl3)
S. Barathiraja*, Lijo John, N. Vijay, P. A. V. Gangadhara and G. R. Reddy
Department of Veterinary Biochemistry, Rajiv Gandhi Institute of Veterinary Education and Research (RIVER), Kurumbapet, Puducherry- 605 009, India
*Corresponding author
Abstract:

Type III Interferons also known as Interferon lambdas (IFNs/IFNLs) constitute a recently described IFN with antiviral activities by signaling through a unique receptor complex composed of IFNs receptor 1 (IFNR1) and interleukin-10 receptor 2 (IL10R2). It also found to have more of immune modulatory activities in addition to antiviral activities. In the present study, the construct pcDNA-bovine IFNλ3 was administered intramuscularly to mice. The mice were sacrificed on 7th and 14th day to collect the different organs/tissues. The tissues were used to isolate total RNA and following cDNA conversion. Then, the tissues were analyzed for the expression of IFN gene by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). The results had shown that there was expression of given IFNλ3 in organs: leg muscle, liver and spleen and also in blood.


Keywords: Bovine Interferon Lambda3; pcDNA- IFNλ3, Mice; RT-PCR
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How to cite this article:

Barathiraja, S., Lijo John, N. Vijay, P. A. V. Gangadhara and Reddy, G. R. 2019. Expression of pcDNA-Interferon LAMBDA-3 (pcDNA-IFN3).Int.J.Curr.Microbiol.App.Sci. 8(9): 2585-2589. doi: https://doi.org/10.20546/ijcmas.2019.809.299