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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 2042-2051
DOI: https://doi.org/10.20546/ijcmas.2019.809.236


GC-MS Analysis of Bioactive Compounds of Seaweed Extracts Collected from Seashore of Manalmelkudi (Pudukkottai dist., Tamilnadu), responsible for Antifungal Activity
K. G. Anitha*, G. Arputha, G. Muthubala, R. Susithra, M. Mullaivendhan and R. Anandham
Agricultural College and Research Institute, Tamil Nadu Agricultural University, Kudumiyanmalai - 622 104, Pudukkottai, Tamil Nadu, India
*Corresponding author
Abstract:

The antifungal activity of Gracilaria cervicornis, Gracilaria gracilis, Endocladia muricata is tested against Macrophomina phaseolina and Lasiodiplodia theobromae. The mean inhibion zones induced by seaweed extracts on the tested pathogens revealed that methanolic extract of G. cervicornis had highest zone than its acetone & aquous extracts followed by methanoilc extract of G. gracilis against M. phaseolina whereas no inhibition by E. muricata. The methanolic extract of G. cervicornis recorded inhibition zone of 20.7 mm against M. phaseolina whereas in L. theobromae it was 17.6 mm. But the acetone extract of G. gracilis showed 17.4 mm against M. phaseolina whereas it was 16.6 mm for G. cervicornis. The composition of bioactive compounds in the GC-MS chromatogram of these seaweed extracts were analysed and found that the phenols contributed major portion among the various fractions of the extract which contributes for the antimicrobial effect. The peaks for phenols with area % of 38.53 and height % of 28.58 for G. cervicornis and 32.15% and 19.56% respectively for G. gracilis are recorded.The chromatogram of Endocladia muricata showed no traces of phenols. G. gracilis is having appreciable amounts of fatty acids with notable height % viz., Hexadecanoic acid (3.45%), n- Hexadecanoic acid (5.10%), Furanacetic acid (2.3%) and in G. cervicornis Tridecanoicacid, n-Nonadecanoic acid, cyclo propane octanoic acid, Heneicosanoic acid which are also responsible for the exhibited antifungal activity.


Keywords: Seaweed, Antifungal acitivity, bioactive compounds, GC-MS analysis
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How to cite this article:

Anitha, K. G., G. Arputha, G. Muthubala, R. Susithra, M. Mullaivendhan and Anandham, R. 2019. GC-MS Analysis of Bioactive Compounds of Seaweed Extracts Collected from Seashore of Manalmelkudi (Pudukkottai dist., Tamilnadu), responsible for Antifungal Activity.Int.J.Curr.Microbiol.App.Sci. 8(9): 2042-2051. doi: https://doi.org/10.20546/ijcmas.2019.809.236