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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Int.J.Curr.Microbiol.App.Sci.2019.8(9): 1987-1998
DOI: https://doi.org/10.20546/ijcmas.2019.809.230


Characterization of Guava Root Knot Nematode, Meloidogyne enterolobii Occurring in Tamil Nadu, India
P. Suresh1, K. Poornima1*, P. Kalaiarasan1S. Nakkeeran 2 and R. M Vijayakumar3
1Department of Nematology,
2Department of Plant Pathology
3Department of Fruit Crops, Tamil Nadu Agricultural University,Coimbatore, Tamil Nadu-641003, India
*Corresponding author
Abstract:

Root knot nematodes (Meloidogyne spp.) are the most common and destructive plant parasitic nematode group with wide host range of crops, including guava (Psidium guajava L.). Meloidogyne enterolobii (Syn:M. mayaguensis), the root knot nematode of the guava tree, belongs to the group of tropical root knot nematodes and is considered as one of the most damaging species, due to its wide host range, pathogenicity and ability to develop and reproduce on several crops carrying resistance genes. The present study was aimed at identifying Meloidogyne species attacking guava orchards in three districts such as Dindigul, Coimbatore and Thiruvannamalai districts of Tamil Nadu. The morphological study based on perineal patterns of the females confirmed the presence of Meloidogyne enterolobii. In addition, the molecular identification was carried out based on the partial sequence of internal transcribed spacer (ITS1-ITS2) regions. The amplicon size was approximately 600 bp. The sequences were compared with those of M. enterolobii in the GenBank database with high similarity (98%). Sequences were submitted in NCBI and obtained gene accession numbers of Dindigul populations (MK940246 and MK955348), Coimbatore populations (MK955350 and MN006626) and Thiruvannamalai populations (MN381161 and MN381109) were obtained. Phylogenetic studies placed present study population with other Meloidogyne species retrieved from the GenBank database.


Keywords: Guava, root knot nematode, Meloidogyne enterolobii, perineal pattern, ITS and PCR.
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How to cite this article:

Suresh, P., K. Poornima, P. Kalaiarasan, S. Nakkeeran and Vijayakumar, R. M. 2019. Characterization of Guava Root Knot Nematode, Meloidogyne enterolobii Occurring in Tamil Nadu.Int.J.Curr.Microbiol.App.Sci. 8(9): 1987-1998. doi: https://doi.org/10.20546/ijcmas.2019.809.230